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引用 Bioss 抗體： 356支；其中 2 篇文章中引用抗體達10 支；另有，12 篇文章引用 5 至 9支，26 篇文章引用抗體 3 至 5 支。

Institution : 中國科學院上海藥物研究所藥物研究與藥劑學中心國家重點實驗室

摘要：Multivesicular bodies (MVBs) fuse with not only the plasma membranes to release extracellular vesicles (EVs) but also lysosomes for degradation. Rab7 participates in the lysosomal targeting of MVBs. However, the proteins on MVB that directly bind Rab7, causing MVB recruitment of Rab7 remain unidentified. Here, we show that Coro1a undergoes neddylation modification at K233 by TRIM4. Neddylated Coro1a is associated with the MVB membrane and facilitates MVB recruitment and activation of Rab7 by directly binding Rab7. Subsequently, MVBs are targeted to lysosomes for degradation in a Rab7-dependent manner, leading to reduced EV secretion. Furthermore, a decrease in neddylated Coro1a enhances the production of tumour EVs, thereby promoting tumour progression, indicating that neddylated Coro1a is an ideal target for the regulation of EV biogenesis. Altogether, our data identify a novel substrate of neddylation and reveal an unknown mechanism for MVB recruitment of Rab7, thus providing new insight into the regulation of EV biogenesis.

Pubmed ID : 34719860

文獻引用抗體：bs-0813R | Anti-casein pAb | FC

Institution : 福建省廈門市集美大學食品與生物工程學院生物工程系

摘要：Extracellular vesicles (EVs) have demonstrated unique advantages in serving as nanocarriers for drug delivery, yet the cargo encapsulation efficiency is far from expectation, especially for hydrophilic chemotherapeutic drugs. Besides, the intrinsic heterogeneity of EVs renders it difficult to evaluate drug encapsulation behaviour. Inspired by the active drug loading strategy of liposomal nanomedicines, here we report the development of a method, named “Sonication and Extrusion-assisted Active Loading” (SEAL), for effective and stable drug encapsulation of EVs. Using doxorubicin-loaded milk-derived EVs (Dox-mEVs) as the model system, sonication was applied to temporarily permeabilize the membrane, facilitating the influx of ammonium sulfate solution into the lumen to establish the transmembrane ion gradient essential for active loading. Along with extrusion to downsize large mEVs, homogenize particle size and reshape the nonspherical or multilamellar vesicles, SEAL showed around 10-fold enhancement of drug encapsulation efficiency compared with passive loading. Single-particle analysis by nano-flow cytometry was further employed to reveal the heterogeneous encapsulation behaviour of Dox-mEVs which would otherwise be overlooked by bulk-based approaches. Correlation analysis between doxorubicin auto-fluorescence and the fluorescence of a lipophilic dye DiD suggested that only the lipid-enclosed particles were actively loadable. Meanwhile, immunofluorescence analysis revealed that more than 85% of the casein positive particles was doxorubicin free. These findings further inspired the development of the lipid-probe- and immuno-mediated magnetic isolation techniques to selectively remove the contaminants of non-lipid enclosed particles and casein assemblies, respectively. Finally, the intracellular assessments confirmed the superior performance of SEAL-prepared mEV formulations, and demonstrated the impact of encapsulation heterogeneity on therapeutic outcome. The as-developed cargo-loading approach and nano-flow cytom.

Pubmed ID : 34708571

文獻引用抗體：bs-5884R | Anti-Endomucin pAb | IHC

Pubmed ID : 34719890

文獻引用抗體：bsm-33283M | Mouse Anti-Bax mAb | IHC

Institution : 廣東省口腔疾病診療工程研究中心北京大學深圳醫(yī)院廣東省高水平臨床重點專科口腔頜面外科

摘要：Chemodynamic therapy (CDT) is an emerging treatment that usually employs chemical agents to decompose hydrogen peroxide (H?O?) into hydroxyl radical (?OH) via Fenton or Fenton-like reactions, inducing cell apoptosis or necrosis by damaging biomacromolecules such as, lipids, proteins, and DNA. Generally, CDT shows high tumor-specificity and minimal-invasiveness in patients, thus it has attracted extensive research interests. However, the catalytic reaction efficiency of CDT is largely limited by the relatively high pH at the tumor sites. Herein, a 808 nm laser-potentiated peroxidase catalytic/mild-photothermal therapy of molybdenum diphosphide nanorods (MoP? NRs) is developed to improve CDT performance, and simultaneously achieve effective tumor eradication and anti-infection. In this system, MoP? NRs exhibit a favorable cytocompatibility due to their inherent excellent elemental biocompatibility. Upon irradiation with an 808 nm laser, MoP? NRs act as photosensitizers to efficiently capture the photo-excited band electrons and valance band holes, exhibiting enhanced peroxidase-like catalytic activity to sustainedly decompose tumor endogenous H?O? to ?OH, which subsequently destroy the cellular biomacromolecules both in tumor cells and bacteria. As demonstrated both in vitro and in vivo, this system exhibits a superior therapeutic efficiency with inappreciable toxicity. Hence, the work may provide a promising therapeutic technique for further clinical applications.

Pubmed ID : 34635817

文獻引用抗體：bsm-33235M | Mouse Anti-alpha Tubulin (Acetyl Lys40) mAb | IF

Institution : 南京醫(yī)科大學生殖醫(yī)學國家重點實驗室

摘要：Many integral membrane proteins might act as indispensable coordinators in specific functional microdomains to maintain the normal operation of known receptors, such as Notch. Gm364 is a multi-pass transmembrane protein that has been screened as a potential female fertility factor. However, there have been no reports to date about its function in female fertility. Here, we found that global knockout of Gm364 decreased the numbers of primordial follicles and growing follicles, impaired oocyte quality as indicated by increased ROS and γ-H2AX, decreased mitochondrial membrane potential, decreased oocyte maturation, and increased aneuploidy. Mechanistically, Gm364 directly binds and anchors MIB2, a ubiquitin ligase, on the membrane. Subsequently, membrane MIB2 ubiquitinates and activates DLL3. Next, the activated DLL3 binds and activates Notch2, which is subsequently cleaved within the cytoplasm to produce NICD2, the intracellular active domain of Notch2. Finally, NICD2 can directly activate AKT within the cytoplasm to regulate oocyte meiosis and quality.

Pubmed ID : 34602091

文獻引用抗體：bs-0737R | Anti-HIF-1α pAb | FC

Institution : 揚州大學動物科學與技術學院動物生理與分子營養(yǎng)實驗室

摘要：Background: Intestinal Peyer’s patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium Limosilactobacillus reuteri R2LC affects B lymphocytes and IgA induction in the PPs, as well as the downstream consequences on intestinal microbiota and susceptibility to inflammation. Results: The B cells of PPs were separated by size to circumvent activation-dependent cell identification biases due to dynamic expression of markers, which resulted in two phenotypically, transcriptionally, and spatially distinct subsets: small IgD⁺/GL7^?/S1PR1⁺/Bcl6, CCR6-expressing pre-germinal center (GC)-like B cells with innate-like functions located subepithelially, and large GL7⁺/S1PR1^?/Ki67⁺/Bcl6, CD69-expressing B cells with strong metabolic activity found in the GC. Peroral L. reuteri administration expanded both B cell subsets and enhanced the innate-like properties of pre-GC-like B cells while retaining them in the sub-epithelial compartment by increased sphingosine1-phosphate/S1PR1 signaling. Furthermore, L. reuteri promoted GC-like B cell differentiation, which involved expansion of the GC area and autocrine TGFβ-1 activation. Consequently, PD-1-T follicular helper cell-dependent IgA induction and production was increased by L. reuteri, which shifted the intestinal microbiome and protected against dextran-sulfate-sodium induced colitis and dysbiosis. Conclusions: The Peyer’s patches sense, enhance and transmit probiotic signals by increasing the numbers and effector functions of distinct B cell subsets, resulting in increased IgA production, altered intestinal microbiota, and protection against inflammation.

Institution : 中國核工業(yè)集團公司成都醫(yī)學院第二附屬醫(yī)院416醫(yī)院

摘要：As an innovative treatment strategy for cancer treatment, Fenton reaction-mediated Chemodynamic therapy (CDT) dependent on the conversion of endogenous hydrogen peroxide (H?O?) into cytotoxic hydroxyl radical (?OH) to destroy tumor cells has attracted increasing interest. However, the insufficient supply of H?O? has greatly hindered the anticancer efficacy of CDT in the tumor microenvironment, and this inherent disadvantage has rarely attracted attention. In order to develop a strategy with high-efficiency H?O? self-supply ability to enhance the CDT efficacy, we constructed a Fe-based metal–organic framework (MOF) as a carrier for the drug Juglone (JUG), which can not only increase the intracellular H?O? concentration, but also serve as an inhibitor of the peptidyl-prolyl cis–trans isomerase NIMA-interacting 1 (Pin1), which is a key regulator for multiple physiological processes in cancer cells. Once internalized by tumor cells, the acidic environment of the tumor promotes the release of JUG for drug therapy, and the released Fe ions trigger the Fenton reaction to produce ?OH from sufficient H?O?, thereby achieving significant antitumor effects. Furthermore, the coating of cancer cell membranes onto the JUG-loaded MOF could enable it with homologous tumor targeting ability. Both in vitro and in vivo results show that the use of drugs can activate the cascade to provide sufficient H?O? with outstanding anti-tumor efficacy, which paves a new avenue for the realization of drug/CDT synergistic therapy.