| 產(chǎn)品編號 | bs-1207R |
| 英文名稱 | Rabbit Anti-GLUT3 antibody |
| 中文名稱 | 葡萄糖轉(zhuǎn)運蛋白3抗體 |
| 別 名 | facilitated glucose transporter member 3; Glucose Transporter GLUT3 ; FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT-3; GTR3_HUMAN; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3; Solute carrier family 2. |
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Specific References (6) | bs-1207R has been referenced in 6 publications.
[IF=5.47] Yan, Yu-E., et al. ?Significant Reduction of the GLUT3 Level, but not GLUT1 Level, Was Observed in the Brain Tissues of Several Scrapie Experimental Animals and Scrapie-Infected Cell Lines.? Molecular neurobiology (2013): 1-14. WB ;
[IF=5.076] Hu C et al. Placentae for Low Birth Weight Piglets Are Vulnerable to Oxidative Stress, Mitochondrial Dysfunction, and Impaired Angiogenesis. Oxid Med Cell Longev. 2020 May 25;2020:8715412. WB ; Pig.
[IF=3.195] Huang, Hui. et al. Osthole increases the radiosensitivity of hepatoma cells by inhibiting GSK-3β/AMPK/mTOR pathway-controlled glycolysis. N-S ARCH PHARMACOL. 2022 Nov;:1-10 WB ; Human.
[IF=3.166] Yun S et al. Effects of lead exposure on brain glucose metabolism and insulin signaling pathway in the hippocampus of rats. Toxicol Lett. 2019 Aug;310:23-30. WB ; Rat.
[IF=3.064] Zhao, Ying. et al. Apigenin increases radiosensitivity of glioma stem cells by attenuating HIF-1α-mediated glycolysis. Med Oncol. 2021 Nov;38(11):1-10 WB ; human.
[IF=2.136] ?Yu?CL et al. AMP-activated protein kinase negatively regulates heat treatment-induced lactate secretion in cultured boar sertoli cells. Theriogenology. 2018 Nov;121:35-41. WB ; boar.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLUT3: 151-260/493 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
may act as a glucose transporter in neurons; may mediate increasd glucose uptake in response to neuronal injury. Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis. Alternative names: FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT3; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3. Function: Facilitative glucose transporter. Probably a neuronal glucose transporter. Subcellular Location: Cell membrane; Multi-pass membrane protein. Melanosome. Tissue Specificity: Highly expressed in brain. Expressed in many tissues. Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. SWISS: P11169 Gene ID: 6515 Database links: Entrez Gene: 6515 Human Entrez Gene: 20527 Mouse Omim: 138170 Human SwissProt: P11169 Human SwissProt: P32037 Mouse Unigene: 419240 Human Unigene: 395108 Mouse Unigene: 95055 Rat 交換和轉(zhuǎn)運（Trafficking and Transport） GLUT-3屬于溶質(zhì)運載蛋白家族成員（solute carrier family），主要功能是轉(zhuǎn)載葡萄糖進入上皮細胞。 目前主要用于糖尿病腎病和視網(wǎng)膜病變的研究，也是腎小球系膜細胞上的主要葡萄糖轉(zhuǎn)運體。GLUT3的功能狀態(tài)直接影響系膜細胞的糖代謝及功能變化。 近期，研究人員也用來區(qū)別一些良、惡性腫瘤的鑒別。 |
| 產(chǎn)品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
293T (Human) Cell Lysate at 30 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
Primary: Anti-GLUT3 (bs-1207R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 50/63 kD
Sample:
293T(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-bs-1207R? (bs-1207R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 63 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated(bs-1207R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, FITC conjugated(bs-1207R-FITC) 1:100, 60 minutes at 37°C;
Excitation wavelength: 488nm; Emission wavelength:519nm
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated(bs-1207R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
