| 產(chǎn)品編號 | bs-3521R |
| 英文名稱 | Rabbit Anti-EZH2 antibody |
| 中文名稱 | 抑癌蛋白EZH2抗體 |
| 別 名 | Enhancer of zeste homolog 2; Enx1h; MGC9169; Enhancer of zeste 2; ENX-1; ENX 1; ENX1; EZH 2; EZH2_HUMAN; Histone-lysine N-methyltransferase EZH2; KMT6A; Lysine N-methyltransferase 6; Enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste, Drosophila, homolog 2; KMT 6;KMT6A; KMT6; WVS2. |
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Specific References (3) | bs-3521R has been referenced in 3 publications.
[IF=8.786] Chengmei He. et al. EZH2 Promotes T Follicular Helper Cell Differentiation Through Enhancing STAT3 Phosphorylation in Patients With Primary Sj?gren’s Syndrome. FRONT IMMUNOL. 2022; 13: 922871 IHC ; Human.
[IF=5.201] Wang Shanshan. et al. RIP-Seq of EZH2 Identifies TCONS-00036665 as a Regulator of Myogenesis in Pigs. Front Cell Dev Biol. 2021 Jan;8:1682 IP ; Pig.
[IF=5.076] Wang Peng. et al. ALKBH5 Exacerbates Aortic Dissection by Promoting Inflammatory Response and Apoptosis of Aortic Smooth Muscle Cells via Regulating lnc-TMPO-AS1/EZH2/IRAK4 Signals in an m6A Modification Manner. Oxid Med Cell Longev. 2021;2021:5513966 IF ; Human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 82kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KMT6/EZH2: 255-350/746 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]. Function: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4. Subunit: Binds ATRX via the SET domain (Probable). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Subcellular Location: Nucleus. Tissue Specificity: Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. Post-translational modifications: Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Similarity: Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 CXC domain. Contains 1 SET domain. SWISS: Q15910 Gene ID: 2146 Database links: Entrez Gene: 2146 Human Entrez Gene: 14056 Mouse Omim: 601573 Human SwissProt: Q15910 Human SwissProt: Q61188 Mouse Unigene: 444082 Human Unigene: 246688 Mouse Unigene: 9027 Rat ????EZH2（enhancer of zeste homolog 2）是新識別的一種人類基因，是果蠅zeste基因增強(qiáng)子的人類同源基因，Polycomb group基因家族的重要成員之一。 ????EZH2在多種腫瘤中高表達(dá)，具有促進(jìn)細(xì)胞增殖，腫瘤細(xì)胞的擴(kuò)散的惡性表型，EZH2在腫瘤中的作用成為研究熱門。EZHZ在前列腺癌、乳腺癌、膀朧癌、肝細(xì)胞癌、大腸癌、胃癌中高表達(dá);局限性前列腺癌、乳腺癌、膀朧癌高表達(dá)EZH2，則臨床預(yù)后較差。因此EZH2可作為腫瘤標(biāo)志物監(jiān)測腫瘤的演變。隨著PcG蛋白和TrxG蛋白復(fù)合物研究的深入，對細(xì)胞記憶機(jī)制的認(rèn)識不斷提高，而擾亂細(xì)胞的轉(zhuǎn)錄記憶系統(tǒng)可導(dǎo)致個體發(fā)育缺陷，使正常細(xì)胞丟失自身特性而癌變。 ????EZH2通過基因沉默機(jī)制改變細(xì)胞的記憶系統(tǒng)并調(diào)控轉(zhuǎn)錄，促進(jìn)前腫瘤惡性形成。EZH2在腫瘤發(fā)生發(fā)展中的作用仍遠(yuǎn)未闡明,特別是EZH2的下游基因仍不清楚,EZH2在腫瘤發(fā)生與發(fā)展的機(jī)制有待進(jìn)一步研究。 |
| 產(chǎn)品圖片 |
Tissue/cell: human gastric cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KMT6 Polyclonal Antibody, Unconjugated(bs-3521R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-KMT6
antibody (bs-3521R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-KMT6 antibody (bs-3521R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-KMT6 antibody (bs-3521R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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