| 產(chǎn)品編號 | bs-3675R |
| 英文名稱 | Rabbit Anti-R-cadherin antibody |
| 中文名稱 | R-鈣粘附分子抗體 |
| 別 名 | Cadherin 4; Cadherin4; Cadherin-4 CDH4; R CAD; Retinal Cadherin. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 激酶和磷酸酶 細胞粘附分子 糖蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 81kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human R-cadherin: 401-500/916 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cadherins constitute a superfamily of calcium-dependent intercellular adhesion molecules and are thought to play a role in cell recognition and segregation, morphogenetic regulation, and tumor suppression. R Cadherin is expressed mainly in the brain but is also found in other tissues. Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed mainly in brain but also found in other tissues. Similarity: Contains 5 cadherin domains. SWISS: P55283 Gene ID: 1002 Database links: Entrez Gene: 1002 Human Entrez Gene: 12561 Mouse Omim: 603006 Human SwissProt: P55283 Human SwissProt: P39038 Mouse SwissProt: Q80ZV4 Mouse Unigene: 473231 Human Unigene: 598638 Human Unigene: 184711 Mouse Unigene: 382094 Mouse Unigene: 392073 Mouse Unigene: 214089 Rat R-鈣粘附分子,是依賴鈣離子的細胞間糖蛋白粘附分子的一種，主要是參與細胞間同源性粘附、調(diào)控和介導(dǎo)細胞間相互反應(yīng)，在腫瘤的遷移、分化中起到一定的作用。在腦組織中表達外，其他組織中已有定位。 |
| 產(chǎn)品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-R-cadherin/Cadherin 4 Polyclonal Antibody, Unconjugated(bs-3675R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
