| 產(chǎn)品編號 | bs-2352R |
| 英文名稱 | Cytochrome P450 2B6 Rabbit pAb |
| 中文名稱 | 細胞色素P450 2B6抗體 |
| 別 名 | 11B1; CP2B6_HUMAN; CPB 6; CPB6; CYP 2B6; CYP IIB6; CYP2B; CYP2B6; CYP2B7; CYP2B7P; CYPIIB6; Cytochrome P450 2B6; Cytochrome P450 family 2 subfamily B; Cytochrome P450 family 2 subfamily B polypeptide 6; Cytochrome P450 IIB1; Cytochrome P450 phenobarbital inducible; Cytochrome P450 subfamily IIB(phenobarbital inducible); Cytochrome P450 subfamily IIB(phenobarbital inducible) polypeptide 6; Cytochrome P450 subfamily IIB polypeptide 6; 1, 4-cineole 2-exo-monooxygenase; IIB1; P450; P450 IIB1; P450IIB1. |
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Specific References (1) | bs-2352R has been referenced in 1 publications.
[IF=2.8] Man, Shuli, et al. "Combination therapy of cyclophosphamide and Rhizoma Paridis Saponins on anti-hepatocarcinoma mice and effects on cytochrome p450 enzyme expression." Steroids (2013). WB ; Rat.
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| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 信號轉導 細胞凋亡 轉錄調(diào)節(jié)因子 新陳代謝 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cytochrome P450 2B6: 401-491/491 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Tissue Specificity: Expressed in liver, lung and heart right ventricle. Post-translational modifications: Phosphorylation is accompanied by a decrease in enzyme activity (By similarity). Similarity: Belongs to the cytochrome P450 family. SWISS: P20813 Gene ID: 1555 Database links: Entrez Gene: 1555 Human Omim: 123930 Human SwissProt: P20813 Human Unigene: 1360 Human |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome P450 2B6 (bs-2352R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome P450 2B6 (bs-2352R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Cytochrome P450 2B6 antibody (bs-2352R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
