| 產(chǎn)品編號 | bs-3996R |
| 英文名稱 | MDH1 Rabbit pAb |
| 中文名稱 | 可溶性蘋果酸脫氫酶抗體 |
| 別 名 | Cytosolic malate dehydrogenase; Malate dehydrogenase 1, NAD(soluble); MDHA; MDHs; Soluble malate dehydrogenase; Malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1; Malate dehydrogenase 1 NAD(soluble); Malate dehydrogenase 2; Malate dehydrogenase cytoplasmic; Malate dehydrogenase 2 NAD(mitochondrial); MDH 1; MDH 2; MDH; MDH2; MDHA; MDHm; MDHs; MMDH; Mor 1; Mor 2; Mor1; MOR2; cytoplasmic; MDH s; MDHC_HUMAN; MGC:1375; SMDH; Soluble malate dehydrogenase. |
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Specific References (2) | bs-3996R has been referenced in 2 publications.
[IF=19.328] Patricia Altea-Manzano. et al. Reversal of mitochondrial malate dehydrogenase 2 enables anaplerosis via redox rescue in respiration-deficient cells. MOL CELL. 2022 Nov;: WB ; Human.
[IF=6.1] Paul, Subhojit, et al. "STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO 2 gradient." Redox Biology (2017). WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MDH1: 265-334/334 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. Malate dehydrogenase 1 (MDH1) is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Subunit: Homodimer. Subcellular Location: Cytoplasm. Post-translational modifications: ISGylated. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation. Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family. SWISS: P40925 Gene ID: 4190 Database links: Entrez Gene: 4190 Human Entrez Gene: 17449 Mouse Omim: 154200 Human SwissProt: P40925 Human SwissProt: P14152 Mouse Unigene: 526521 Human Unigene: 212703 Mouse Unigene: 13492 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Stomach tissue lysates
Lane 5: Rat Heart tissue lysates
Lane 6: Rat Cerebrum tissue lysates
Lane 7: Rat Stomach tissu
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation wit
Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-MDH1 antibody (bs-3996)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-DMT1 antibody (bs-3996R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-
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