| 產(chǎn)品編號 | bs-5280R |
| 英文名稱 | phospho-CTNNA1 (Ser641) Rabbit pAb |
| 中文名稱 | 磷酸化α-連環(huán)蛋白抗體 |
| 別 名 | p-CTNNA1(Ser641); CTNNA1(phospho-Ser641); CTNNA1(phospho-S641); alpha 1 Catenin(phospho S641); alpha catenin; alpha E catenin; alphaE catenin; Cadherin associated protein 102kDa; Cadherin associated protein; CAP 102; CAP102; Catenin(cadherin associated protein) alpha 1 102kDa; Catenin alpha 1; CTNNA 1; CTNNA1; alpha-cats; FLJ36832; NY REN 13 antigen; CTNA1_HUMAN. Catenin α; Catenin-α; Catenin-α1; Catenin α1; α Catenin; 鈣粘蛋白相關(guān)蛋白; |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 干細(xì)胞 細(xì)胞粘附分子 細(xì)胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 100 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human CTNNA1 around the phosphorylation site of Ser641: DD(p-S)DF |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. Function: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. Subunit: Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds MLLT4 and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma-catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1. Subcellular Location: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries. Tissue Specificity: Expressed ubiquitously in normal tissues. Post-translational modifications: Sumoylated. Similarity: Belongs to the vinculin/alpha-catenin family. SWISS: P35221 Gene ID: 1495 Database links: Entrez Gene: 1495 Human Entrez Gene: 12385 Mouse Omim: 116805 Human SwissProt: P35221 Human SwissProt: P26231 Mouse α-catenin是一種多功能的蛋白質(zhì)，能鏈接E-\N-\P-鈣粘附分子,具有參與細(xì)胞粘附和介導(dǎo)信號轉(zhuǎn)導(dǎo)的雙重功能，并與腫瘤的發(fā)生發(fā)展及浸潤密切相關(guān)。 在正常個體中，βα-catenin和鈣黏蛋白形成復(fù)合體，介導(dǎo)同型細(xì)胞的粘附，維持細(xì)胞的穩(wěn)定；同時，α-catenin作為Wnt/α-catenin信號通路的關(guān)鍵成員在介導(dǎo)信號轉(zhuǎn)導(dǎo)過程中調(diào)控細(xì)胞的增殖和凋亡。 在惡性腫瘤中，α-catenin的表達(dá)呈現(xiàn)明顯的異質(zhì)性，促使細(xì)胞異常增殖，還可使細(xì)胞之間的黏附性減弱，侵襲性增強(qiáng)。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30
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