| 產品編號 | bs-6168R |
| 英文名稱 | Rabbit Anti-RAB8B antibody |
| 中文名稱 | ras癌基因家族RAB8B抗體 |
| 別 名 | GTPase Rab8b; 5930437D16; FLJ38125; RAB 8b protein; RAB44; Rab8b; RAB8B, member RAS oncogene family; RAB8B_HUMAN; Ras-related protein Rab-8B. |
| 研究領域 | 腫瘤 細胞生物 染色質和核信號 信號轉導 G蛋白偶聯(lián)受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RAB8B: 21-120/207 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
RAB proteins, like RAB8B, are low molecular mass monomeric GTPases that localize on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB proteins function in intracellular vesicle transport by aiding in the docking and/or fusion of vesicles with their target membranes Function: May be involved in vesicular trafficking and neurotransmitter release (By similarity). May participate in cell junction dynamics in Sertoli cells. Subunit: Interacts with OTOF and PEX5R (By similarity). Interacts with RAB3IP. Interacts with VIM AND CDH1 (By similarity). Associated with actin, delta-catenin and alpha and beta tubulins. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: Q92930 Gene ID: 51762 Database links: Entrez Gene: 51762 Human Entrez Gene: 235442 Mouse Omim: 613532 Human SwissProt: Q92930 Human SwissProt: P61028 Mouse Unigene: 389733 Human Unigene: 260376 Mouse Unigene: 10995 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAB8B) Polyclonal Antibody, Unconjugated (bs-6168R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
