| 產(chǎn)品編號(hào) | bs-6685R |
| 英文名稱 | Rabbit Anti-SCN10A/Nav1.8 antibody |
| 中文名稱 | 鈉通道蛋白10α抗體 |
| 別 名 | mPN3; Peripheral nerve sodium channel 3; Pn3 (gene name)v PN3; Scn10a; Sensory neuron sodium channel; Sns (gene name); SNS; sodium channel protein type 10 subunit alpha; Sodium channel protein type X alpha subunit; Voltage-gated sodium channel alpha subunit Nav1.8; NAV1.8. |
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Specific References (1) | bs-6685R has been referenced in 1 publications.
[IF=3.688] Tingting Zhang. et al. Mechanism Insight into the in situ Reactions of Repeated Intramuscular Progesterone Injections. BASIC CLIN PHARMACOL. 2022 Sep;: IHC ; Rat.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 通道蛋白 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 215kDa |
| 細(xì)胞定位 | 細(xì)胞膜 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SCN10A/NAV1.8: 1151-1250/1956 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nav1.8 protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, Nav1.8 forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Nav1.8 plays a role in neuropathic pain mechanisms. Function: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Its electrophysiological properties vary depending on the type of the associated beta subunits (in vitro). Plays a role in neuropathic pain mechanisms. Subunit: The voltage-resistant sodium channel consists of an ion conducting pore forming alpha-subunit regulated by one or more associated auxiliary subunits SCN1B, SCN2B and SCN3B. Found in a number of complexes with PRX, DYNLT1 and PDZD2. Interacts with proteins such as FSTL1, PRX, DYNLT1, PDZD2, S100A10 and many others. Interacts with NEDD4 and NEDD4L. Subcellular Location: Membrane; Multi-pass membrane protein. Note=It can be translocated to the extracellular membrane through association with S100A10. Tissue Specificity: Expressed in the dorsal root ganglia and sciatic nerve. Post-translational modifications: Ubiquitinated by NEDD4L; which promotes its endocytosis. Similarity: Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily. Contains 1 IQ domain. SWISS: Q9Y5Y9 Gene ID: 6336 Database links: Entrez Gene: 6336 Human Entrez Gene: 20264 Mouse Omim: 604427 Human SwissProt: Q9Y5Y9 Human SwissProt: P70276 Mouse SwissProt: Q6QIY3 Mouse Unigene: 250443 Human Unigene: 10246 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SCN10A Polyclonal Antibody, Unconjugated(bs-6685R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SCN10A Polyclonal Antibody, Unconjugated(bs-6685R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
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