| 產品編號 | bs-2961R |
| 英文名稱 | Rabbit Anti-Calsequestrin antibody |
| 中文名稱 | 肌鈣集蛋白/收鈣素抗體 |
| 別 名 | CASQ; Calsequestrin 1; Calsequestrin 1 fast twitch skeletal muscle; Calsequestrin 2; Calsequestrin 2 fast twitch cardiac muscle; Calsequestrin cardiac muscle isoform; Calsequestrin skeletal muscle isoform; Cardiac calsequestrin 2; CASQ 1; CASQ 2; PDIB1; PDIB2; Skeletal muscle calsequestrin 1. |
| 研究領域 | 心血管 信號轉導 轉錄調節(jié)因子 結合蛋白 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calsequestrin: 75-170/396 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium. Function: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+). Subcellular Location: Sarcoplasmic reticulum lumen. Mitochondrion. Note=This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial according to PubMed:7945294. Similarity: Belongs to the calsequestrin family. SWISS: P31415 Gene ID: 844 Database links: Entrez Gene: 844 Human Entrez Gene: 12372 Mouse Omim: 114250 Human SwissProt: P31415 Human SwissProt: O09165 Mouse Unigene: 632476 Human Unigene: 12829 Mouse Unigene: 159963 Rat 肌鈣集蛋白，隱鈣素，收鈣素，是肌漿網上調控鈣儲存的主要結合蛋白。CASQ與內質網鈣結合蛋白肌鈣網蛋白具有同源性（calreticulin）。CASQ1主要在快收縮骨骼肌表達，CASQ2主要在在慢收縮骨骼肌和心肌表達。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
