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Rabbit Anti-Tissue factor  antibody (bs-4690R)  
~~~促銷代碼KT202411~~~
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產品編號 bs-4690R
英文名稱 Rabbit Anti-Tissue factor  antibody
中文名稱 組織因子(CD142)抗體
別    名 CD 142; CD142; CD142 antigen; Coagulation factor III (thromboplastin tissue factor); Coagulation factor III; F3; TF; TFA; Thromboplastin; Tissue factor; TF_HUMAN.  
Specific References  (6)     |     bs-4690R has been referenced in 6 publications.
[IF=23.394] Fletcher, Russell B., et al. "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." Cell Stem Cell (2017).  FCM ;  Mouse.  
[IF=7.13] Gleeson, Birgitta M., et al. "Bone marrow‐derived mesenchymal stem cells have innate procoagulant activity and cause microvascular obstruction following intracoronary delivery: Amelioration by anti‐thrombin therapy." STEM CELLS (2015).  Pig.  
[IF=6.048] Takabayashi T et al. Increased expression of L‐plastin in nasal polyp of patients with nonsteroidal anti‐inflammatory drug exacerbated respiratory disease.(2018) Allergy  IHC ;  Human.  
[IF=4.249] Zha C et al. Anti-β2GPI/β2GPI induces neutrophil extracellular traps formation to promote thrombogenesis via the TLR4/MyD88/MAPKs axis activation.Neuropharmacology. 2018 Aug;138:140-150.  ICF ;  Human.  
[IF=2.98] Milano, M., et al. "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." Journal of Applied Toxicology 36.1 (2016): 151-160  WB ;  Rat.  
[IF=2.65] Wang Shijun. et al. Investigations on the Changes of Serum Proteins in Rabbits after Trimeresurus stejnegeri Venom Injection via Mass Spectrometry-Based Proteomics. EVID-BASED COMPL ALT. 2022;2022:9239662  WB ;  Rabbit.  
研究領域 細胞生物  生長因子和激素  細胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,GuineaPig,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 29kDa
細胞定位 細胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Tissue factor: 32-100/295 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Function:
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Subunit:
Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.

Subcellular Location:
Isoform 1: Membrane; Single-pass type I membrane protein.
Isoform 2: Secreted.

Tissue Specificity:
Lung, placenta and pancreas.

Similarity:
Belongs to the tissue factor family.

SWISS:
P13726

Gene ID:
2152

Database links:

Entrez Gene: 2152 Human

Omim: 134390 Human

SwissProt: P13726 Human

Unigene: 62192 Human



產品圖片
Sample: Lane 1: Mouse Lung tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Human A431 cell lysates Lane 4: Human MDA-MB-231 cell lysates Lane 5: Human SiHa cell lysates Primary: Anti-Tissue factor (bs-4690R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Polyclonal Antibody, Unconjugated (bs-4690R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated(bs-4690R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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