| 產(chǎn)品編號 | bs-7106R |
| 英文名稱 | Rabbit Anti-NUPR1 antibody |
| 中文名稱 | 核蛋白p8抗體 |
| 別 名 | Candidate of metastasis 1; COM1; Nuclear protein 1; NUPR1; NUPR1_HUMAN; p8 protein; Protein p8. |
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Specific References (5) | bs-7106R has been referenced in 5 publications.
[IF=24.633] Yanxin Li. et al. Decoding the temporal and regional specification of microglia in the developing human brain. Cell Stem Cell. 2022 Mar;: IF ; Mouse,Human.
[IF=7.126] Narzt et al. A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids. (2018) Redox.Biol. 20:467-482 IF ; Human.
[IF=5.402] Li Tan. et al. Age-Related Decline in Expression of Molecular Chaperones Induces Endoplasmic Reticulum Stress and Chondrocyte Apoptosis in Articular Cartilage. Aging Dis. 2020 Oct; 11(5): 1091–1102 IHC ; Monkey.
[IF=5.391] Shiraki M et al. Deficiency?of?stress-associated?gene?Nupr1?increases?bone?volume?by?attenuating?differentiationof?osteoclasts?and?enhancing?differentiation?of?osteoblasts. FASEB J. 2019 May 8:fj201802322RR. IHF ; Mouse.
[IF=2.74] Li Tan. et al. Dietary saturated fatty acid palmitate promotes cartilage lesions and activates the unfolded protein response pathway in mouse knee joints. Plos One. 2021 Feb;16(2):e0247237 IHC ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號 細(xì)胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 9 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NUPR1/p8: 31-82/82 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Could participate in the response to proapoptotic stimuli and promotes cellular growth in a way that helps the tissue counteract diverse injuries. May contribute to the metastatic phenotype.Tissue specificity: Highly expressed in pancreas. Function: Chromatin-binding protein that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Interacts with MSL1 and inhibits its activity on histone H4 'Lys-16' acetylation (H4K16ac). Binds the RELB promoter and activates its transcription, leading to the transactivation of IER3. The NUPR1/RELB/IER3 survival pathway may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. In breast cancer cells, NUPR1 overexpression leads to the activation of PI3K/AKT signaling pathway, CDKN1A/p21 phosphorylation and relocalization from the nucleus to the cytoplasm, leading to resistance to chemotherapeutic agents, such as doxorubicin. Subunit: Monomer. Directly interacts with MSL1 and binds MORF4L1, two components of histone acetyltransferase complex; the interaction with MORF4L1 may be mediated by MSL1. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed, with high levels in liver, pancreas, prostate, ovary, colon, thyroid, spinal cord, trachea and adrenal gland, moderate levels in heart, placenta, lung, skeletal muscle, kidney, testis, small intestine, stomach and lymph node, and low levels in brain, spleen, thymus and bone marrow. Not detected in peripheral blood leukocytes. Post-translational modifications: Phosphorylated in vitro by PKA and CK. Phosphorylation promotes DNA-binding activity. Similarity: Belongs to the NUPR family. SWISS: O60356 Gene ID: 26471 Database links: Entrez Gene: 26471 Human SwissProt: O60356 Human Unigene: 418692 Human Unigene: 513463 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human L-O2 cell lysates
Lane 2: Human U87MG cell lysates
Primary: Anti-NUPR1 (bs-7106R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 11 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUPR1) Polyclonal Antibody, Unconjugated (bs-7106R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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