| 產品編號 | bs-7713R |
| 英文名稱 | Rabbit Anti-MIS12 antibody |
| 中文名稱 | 染色體及有絲分裂蛋白MIS12抗體 |
| 別 名 | hMIS12; homolog of yeast Mis12; KNTC2AP; MIND kinetochore complex component, homolog (yeast); MIS12 homolog (yeast); MIS12 homolog; MIS12 MIND kinetochore complex component homolog (yeast); MTW 1; MTW1; Protein MIS12 homolog; MIS12_HUMAN. |
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Specific References (3) | bs-7713R has been referenced in 3 publications.
[IF=12.511] Ogasawara et al. Cathepsin K activity controls cardiotoxin-induced skeletal muscle repair in mice. (2018) J.Cachexia.Sarcopenia.Muscle. 9:160-175 IHC-P ; Mouse.
[IF=9.6] Ogasawara, Shinyu, et al. "Cathepsin K activity controls cardiotoxin‐induced skeletal muscle repair in mice." Journal of Cachexia, Sarcopenia and Muscle (2017). IHC-P ; Mouse.
[IF=5.443] Goto Hiroki. et al. Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice. Stem Cells Int. 2021;2021:9202990 IF ; Mouse.
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| 研究領域 | 細胞生物 細胞周期蛋白 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MIS12: 11-120/205 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
MIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. Function: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Subunit: Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Also interacts with CASC5, CBX3, CBX5, NDC80 and ZWINT. Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Note=Associated with the kinetochore. Similarity: Belongs to the mis12 family. SWISS: Q9H081 Gene ID: 79003 Database links: UniProtKB/Swiss-Prot: Q9H081.1 細胞周期是細胞最基本的生命活動之一,它通過一系列有序的反應進行分裂,產生兩個包含相同遺傳物質的子細胞。細胞周期中最核心的事件就是進行遺傳物質的復制和分離,即在S期進行DNA的復制,然后在有絲分裂時期將復制的染色體平均分配到子細胞中去。有絲分裂過程中染色體的運動和分離又是通過紡錘體微管和位于染色體著絲粒上的動點之間的協(xié)同作用來完成的。 MIS12是一個進化上較為保守的蛋白復合物,人源MIS12復合物由Mis13、Mis14、Mis12和PMF1四個蛋白組成,功能性研究表明它們在動點的組裝和染色體的分離中發(fā)揮重要作用,但是，目前它們動點定位機制仍不清楚，還有待進一步研究。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MIS12) Polyclonal Antibody, Unconjugated (bs-7713R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
