產(chǎn)品編號 | bs-7992R |
英文名稱 | Rabbit Anti-CCDC80 antibody |
中文名稱 | 卷曲螺旋結構域蛋白80抗體 |
別 名 | DRO1; okuribin; SSG1; steroid sensitive gene 1; steroid sensitive protein 1; CCD50; CCD80_HUMAN; CCDC80; CL2; Coiled coil domain containing 80; Coiled coil domain containing protein 50; Coiled-coil domain-containing protein 80; Down regulated by oncogenes protein 1; Down-regulated by oncogenes protein 1; Nuclear envelope protein okuribin; Protein Ymer; Up-regulated in BRS-3 deficient mouse homolog; URB. |
Specific References (1) | bs-7992R has been referenced in 1 publications.
[IF=6.684] Jing Wang. et al. Coiled-Coil Domain Containing 80 Suppresses Nonylphenol-Induced Colorectal Cancer Cell Proliferation by Inhibiting the Activation of ERK1/2. Front Cell Dev Biol. 2021; 9: 759820 IHC ; Human.
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研究領域 | 腫瘤 細胞生物 發(fā)育生物學 神經(jīng)生物學 干細胞 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Horse) |
產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 106kDa |
細胞定位 | 細胞外基質 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CCDC80: 201-300/950 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Promotes cell adhesion and matrix assembly. Tissue specificity: Expressed in dermal papilla and dermal fibroblasts (at protein level). Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts. Function: Promotes cell adhesion and matrix assembly (By similarity). Subunit: Binds to various extracellular matrix proteins (By similarity). Subcellular Location: Secreted, extracellular space, extracellular matrix (By similarity). Tissue Specificity: Expressed in dermal papilla and dermal fibroblasts (at protein level). Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts. Post-translational modifications: Phosphorylated. Similarity: Belongs to the CCDC80 family. SWISS: Q76M96 Gene ID: 151887 Database links: Entrez Gene: 151887 Human Entrez Gene: 67896 Mouse SwissProt: Q76M96 Human SwissProt: Q8R2G6 Mouse Unigene: 477128 Human Unigene: 181074 Mouse Unigene: 2193 Rat |
產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC80) Polyclonal Antibody, Unconjugated (bs-7992R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC80) Polyclonal Antibody, Unconjugated (bs-7992R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |