| 產(chǎn)品編號 | bs-4915R |
| 英文名稱 | Rabbit Anti-ACCN1 antibody |
| 中文名稱 | 腦鈉通道蛋白1抗體 |
| 別 名 | ACCN; ASIC2a; BNaC1; ASIC2; ASICs2 ACCN1_HUMAN; Acid sensing ion channel 2; Acid-sensing ion channel 2; Amiloride sensitive brain sodium channel; Amiloride sensitive cation channel 1 neuronal (degenerin); Amiloride sensitive cation channel 1 neuronal; Amiloride-sensitive brain sodium channel; Amiloride-sensitive cation channel 1, neuronal; Amiloride-sensitive cation channel neuronal 1; ASIC2; ASIC2a; BNaC1; Brain sodium channel 1; Degenerin; hBNaC1; Mammalian degenerin homolog; MDEG; Neuronal amiloride sensitive cation channel 1. |
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Specific References (3) | bs-4915R has been referenced in 3 publications.
[IF=8.063] Zhang Y et al. ASIC1a induces synovial inflammation via the Ca2+/NFATc3/RANTES pathway. Theranostics. 2020 Jan 1;10(1):247-264. IHF-P ; Rat.
[IF=4.432] Jing-Wen Su. et al. Estrogen protects against acidosis-mediated articular chondrocyte injury by promoting ASIC1a protein degradation. Eur J Pharmacol. 2021 Jul;:174381 IF ; Rat.
[IF=2.139] T Wang. et al. ASIC1a involves the acid-mediated activation of pancreatic stellate cells associated with autophagy induction. PHYSIOL RES. 2023 Mar 8;72(1):49-57 ICC,WB ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 通道蛋白 淋巴細(xì)胞 t-淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ACCN1: 301-400/512 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Degenerin/epithelial sodium channel (DEG/ENaC) superfamily members are amiloride-sensitive sodium channels that contain intracellular N- and C-termini, 2 two hydrophobic transmembrane regions and a cysteine-containing extracellular loop. Acid sensing ion channel ASIC1, also designated ACCN2, BNAC2 and ASIC1a, is present in brain as a 4.3-kb transcript with localization to rat dorsal root ganglia. In situ hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety. ASIC2, also designated amiloride-sensitive cation channel 1, neuronal (ACCN1), mammalian degenerin, BNAC1 (MDEG) and brain Na+ channel 1, mediates the normal detection of light touch. ASIC2 mRNA is abundant in brain, specifically in neurons. ASIC2 is expressed as 2.7- and 3.7-kb transcripts in brain and spinal cord tissues. ASIC3, also designated ASIC3, SLNAC1 and TNaC1, mediates detection of lasting pH changes and is involved in modulating moderate- to high-intensity pain sensation. ASIC4, also designated ACCN4 and BNAC4, is abundant in pituitary gland and is also present in the inner ear. Function: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. Subcellular Location: Cell membrane. Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. Tissue Specificity: Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary. Similarity: Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN1 subfamily. SWISS: Q16515 Gene ID: 40 Database links: Entrez Gene: 40 Human Entrez Gene: 11418 Mouse Omim: 601784 Human SwissProt: Q16515 Human SwissProt: Q925H0 Mouse Unigene: 368417 Human Unigene: 234998 Mouse Unigene: 37523 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human U-2 OS cell lysates
Lane 4: Human Jurkat cell lysates
Primary: Anti-ACCN1 (bs-4915R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60,63 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ACCN1 Polyclonal Antibody, Unconjugated(bs-4915R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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