| 產(chǎn)品編號 | bs-11660R |
| 英文名稱 | Rabbit Anti-Gbx1 antibody |
| 中文名稱 | 腸和大腦特定同源蛋白1抗體 |
| 別 名 | Gastrulation and brain specific homeobox protein 1; Gastrulation and brain-specific homeobox protein 1; Gastrulation brain homeo box 1; Gastrulation brain homeobox 1; Gbx1; GBX1_HUMAN; Homeobox protein GBX-1; Homeobox protein GBX1. |
| 研究領域 | 腫瘤 染色質(zhì)和核信號 神經(jīng)生物學 信號轉(zhuǎn)導 Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Chicken,Dog,Fruit Fly,Chimpanzee,mpanzee) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Gbx1: 201-300/363 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The homeobox is DNA sequence that is approximately 180 base pairs long that is involved in all steps of the developmental regulation of animals, fungi and plants, from embryogenesis to cell differentiation. The homeobox encodes a protein domain of about 60 amino acids, called the homeodomain, that can bind to DNA. The gastrulation brain homeobox 1 (GBX1) gene, along with another homeobox gene (EN2), maps to chromosome 7q36, and is expressed in septal and pallidal areas and in the basal forebrain cholinergic system, an area implicated in Alzheimer’s disease. The GBX1 gene encodes the GBX1 protein, which localizes to the nucleus and contains 1 homeobox DNA-binding domain. Subcellular Location: Nucleus. Similarity: Contains 1 homeobox DNA-binding domain. SWISS: Q14549 Gene ID: 2636 Database links: Entrez Gene: 2636 Human Entrez Gene: 231044 Mouse Omim: 603354 Human SwissProt: Q14549 Human SwissProt: P82976 Mouse Unigene: 647114 Human Unigene: 246596 Mouse Unigene: 92432 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gbx1) Polyclonal Antibody, Unconjugated (bs-11660R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody:Rabbit Anti-Gbx1 antibody(bs-11660R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
