| 產(chǎn)品編號(hào) | bs-11744R |
| 英文名稱 | Rabbit Anti-Engrailed 1 antibody |
| 中文名稱 | 同源盒蛋白轉(zhuǎn)錄因子EN1抗體 |
| 別 名 | EN1 / Engrailed 1; Engrailed1; En 1; EN1; EN-1; Engrailed; Engrailed homeobox 1; Engrailed homolog 1; HME1_HUMAN; Homeobox protein en-1; Homeobox protein engrailed 1; Homeobox protein engrailed-1; Homeobox protein engrailed-1; Hu-En-1; Mo-en.1. |
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Specific References (1) | bs-11744R has been referenced in 1 publications.
[IF=6.525] Mengfan Wu. et al. Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing. PHARMACEUTICS. 2022 Oct;14(10):2125 IF ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EN1/Engrailed 1: 351-392/392 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The engrailed-1 gene, EN1, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homo-logs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expres-sion of a-synuclein, a gene that is genetically linked to Parkinson’s disease. Subcellular Location: Nucleus. Similarity: Belongs to the engrailed homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q05925 Gene ID: 2019 Database links: Entrez Gene: 2019 Human Entrez Gene: 13798 Mouse Omim: 131290 Human SwissProt: Q05925 Human SwissProt: P09065 Mouse Unigene: 271977 Human Unigene: 2657 Mouse Unigene: 162354 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Muscle (Rat) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Engrailed 1 (bs-11744R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 50 kD
Blank control(blue): Raji Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Engrailed 1/AF647 Conjugated antibody (bs-11744R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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