| 產(chǎn)品編號 | bs-10402R |
| 英文名稱 | Rabbit Anti-phospho-NIFK (Thr238) antibody |
| 中文名稱 | 磷酸化NIFK抗體 |
| 別 名 | NIFK (phospho T238); NIFK (phospho Thr238); p-NIFK(T238); p-NIFK(Thr238); hNIFK; MKI67 FHA Domain Interacting Nucleolar Phosphoprotein; MKI67IP; NOPP34; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI 67; pKi67; MK67I_HUMAN. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Rabbit,Pig,Sheep,Cow,GuineaPig,Horse) |
| 產(chǎn)品應用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human NIFK/MKI67IP around the phosphorylation site of Thr238: VC(p-T)PT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009]. Subunit: Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis. Subcellular Location: Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67. Post-translational modifications: Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: Q8TB66 Gene ID: 84365 Database links: Entrez Gene: 84365 Human Entrez Gene: 67949 Mouse Omim: 611970 Human SwissProt: Q8TB66 Human SwissProt: Q9BYG3 Human SwissProt: Q91VE6 Mouse Unigene: 367842 Human Unigene: 280311 Mouse |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
