| 產(chǎn)品編號 | bs-17000R |
| 英文名稱 | Rabbit Anti-G2E3 antibody |
| 中文名稱 | G2E3蛋白抗體 |
| 別 名 | KIAA1333 FLJ20333; G2/M phase specific E3 ubiquitin protein ligase; G2E3; PHF7B. G2E3_HUMAN |
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Specific References (1) | bs-17000R has been referenced in 1 publications.
[IF=3.253] Shen Yanyan. et al. Comprehensive analysis of the expression, prognostic significance, and regulation pathway of G2E3 in breast cancer. WORLD J SURG ONCOL. 2022 Dec;20(1):1-17 IHC ; Human.
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| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G2E3: 161-250/706 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
KIAA1333 is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.Essential in early embryonic development to prevent apoptotic death. Subcellular Location: Nucleus, nucleolus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm. In the nucleus, delocalizes from the nucleolus to the nucleoplasm in response to DNA damage. Tissue Specificity: Predominantly expressed in brain, liver, kidney, testes and ovary. Similarity: Contains 1 HECT (E6AP-type E3 ubiquitin-proteinligase) domain. Contains 3 PHD-type zinc fingers. SWISS: Q7L622 Gene ID: 55632 Database links: Entrez Gene: 55632 Human Omim: 611299 Human SwissProt: Q7L622 Human SwissProt: Q5RJY2 Mouse |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
