| 產(chǎn)品編號 | bs-17170R |
| 英文名稱 | Rabbit Anti-TPMT antibody |
| 中文名稱 | 硫嘌呤甲基轉移酶抗體 |
| 別 名 | HGNC:12014; S adenosyl L methionine thiopurine S methyltransferase; Thiopurine methyltransferase; Thiopurine S methyltransferase; Thiopurine S-methyltransferase; TPMT; TPMT_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TPMT: 151-245/245 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009] Function: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Subcellular Location: Cytoplasm. DISEASE: Defects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Similarity: Belongs to the methyltransferase superfamily. TPMT family. SWISS: P51580 Gene ID: 7172 Database links: Entrez Gene: 7172 Human Entrez Gene: 22017 Mouse Omim: 187680 Human SwissProt: P51580 Human SwissProt: O55060 Mouse Unigene: 444319 Human Unigene: 10169 Mouse Unigene: 112598 Rat TPMT是硫嘌呤類藥物（如硫唑嘌呤、巰嘌呤和硫鳥嘌呤）的代謝中最主要的酶之一，起到的作用是在這類化合物的硫原子上增加一個甲基；這個過程中提供甲基的是S-腺苷甲硫氨酸，后者同時被轉化成S-腺苷-L-高半胱氨酸。 TPMT基因的缺陷會讓人體無法將這類藥物滅活，未代謝的藥物因此在體內(nèi)大量累積，從而引起嚴重甚至致命的骨髓抑制，表現(xiàn)為貧血、血小板減少癥（導致出血）和白細胞減少癥（導致感染）等. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
