| 產(chǎn)品編號 | bs-17677R |
| 英文名稱 | Rabbit Anti-SPIRE1 antibody |
| 中文名稱 | SPIRE1蛋白抗體 |
| 別 名 | KIAA1135; MGC150621; MGC150622; Protein spire homolog 1; Spir 1; Spir-1; Spir1; SPIR1_HUMAN; SPIRE 1; Spire homolog 1; spire1. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 85kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPIRE1: 651-756/756 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM, Mar 2008] Function: Acts as a actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in vesicle transport processes providing a novel link between actin organization and intracellular transport. Subcellular Location: Cytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Punctate spots in perinuclear region and cytoplasm, co-localised with Rab11. Similarity: Belongs to the spire family. Contains 1 KIND domain. Contains 2 WH2 domains. SWISS: Q08AE8 Gene ID: 56907 Database links: Entrez Gene: 56907 Human Entrez Gene: 68166 Mouse Omim: 609216 Human SwissProt: Q08AE8 Human SwissProt: Q52KF3 Mouse Unigene: 515283 Human Unigene: 208723 Mouse |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
