| 產(chǎn)品編號 | bs-17680R |
| 英文名稱 | Rabbit Anti-phospho-SF1 (Ser82) antibody |
| 中文名稱 | 磷酸化剪接因子1抗體 |
| 別 名 | SF01_HUMAN; Splicing factor 1; ZFM1; ZNF162; Mammalian branch point-binding protein (BBP; mBBP); Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; |
| 研究領域 | 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human SF1 around the phosphorylation site of Ser82: SP(p-S)PE |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] Function: SF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing. Subcellular Location: Nuclear SWISS: Q15637 Gene ID: 7536 Database links: Entrez Gene: 7536 Human Entrez Gene: 22668 Mouse SwissProt: Q15637 Human SwissProt: Q64213 Mouse |
| 產(chǎn)品圖片 |
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-phospho-splicing factor 1 (Ser82) ? (bs-17680R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
