| 產品編號 | bs-19832R |
| 英文名稱 | Rabbit Anti-SLC39A4 antibody |
| 中文名稱 | 溶質載體家族蛋白39成員A4抗體 |
| 別 名 | 1600025H15Rik; Acrodermatitis enteropathica zinc deficiency type; Activated in W/Wv mouse stomach 2; AEZ; AU041686; AWMS2; FLJ20327; MGC156705; MGC74741; S39A4_HUMAN; Slc39a4; Solute carrier family 39 member 4; Ssolute carrier family 39 zinc transporter member 4; Zinc transporter ZIP4; ZIP-4; ZIP4; Zrt and Irt like protein 4; Zrt- and Irt-like protein 4. |
| 研究領域 | 細胞生物 信號轉導 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 66kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC39A4: 281-380/647 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the zinc/iron-regulated transporter-like protein (ZIP) family. The encoded protein localizes to cell membranes and is required for zinc uptake in the intestine. Mutations in this gene result in acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013] Function: Plays an important role in cellular zinc homeostasis as a zinc transporter. Regulated in response to zinc availability. Subcellular Location: Cell membrane. Recycling endosome membrane. Colocalized with TFRC in the recycling endosomes. Cycles between endosomal compartments and the plasma membrane in response to zinc availability. Tissue Specificity: Highly expressed in kidney, small intestine, stomach, colon, jejunum and duodenum. DISEASE: Acrodermatitis enteropathica, zinc-deficiency type (AEZ) Similarity: Belongs to the ZIP transporter (TC 2.A.5) family. SWISS: Q6P5W5 Gene ID: 55630 Database links: Entrez Gene: 55630 Human Entrez Gene: 72027 Mouse Omim: 607059 Human SwissProt: Q6P5W5 Human SwissProt: Q78IQ7 Mouse Unigene: 521934 Human Unigene: 276829 Mouse |
| 產品圖片 |
Sample: MCF-7 (human)Cell Lysate at 40 ug
Primary: Anti- SLC39A4 (bs-19832R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:66kD
Observed band size: 71 kD
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC39A4) Polyclonal Antibody, Unconjugated (bs-19832R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
