| 產(chǎn)品編號 | bsm-10888M |
| 英文名稱 | Mouse Anti-Leptin antibody |
| 中文名稱 | 小鼠抗瘦素單克隆抗體 |
| 別 名 | LEP; Leptin Murine Obesity Homolog; Leptin Precursor Obesity Factor; OB; Obese Protein; Obesity; Obesity factor; Obesity homolog mouse; Obesity Murine Homolog Leptin; OBS; LEP_HUMAN. |
| 研究領域 | 心血管 神經(jīng)生物學 信號轉導 干細胞 生長因子和激素 新陳代謝 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 交叉反應 | Rat (predicted: Human,Mouse) |
| 產(chǎn)品應用 | IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Leptin: 22-167/167 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]. Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Subunit: Interacts with SIGLEC6. Subcellular Location: Secreted (Probable). DISEASE: Leptin deficiency (LEPD) [MIM:614962]: A rare disease characterized by low levels of serum leptin, severe hyperphagia and intractable obesity from an early age. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the leptin family. SWISS: P41159 Gene ID: 3952 Database links: Entrez Gene: 3952 Human Entrez Gene: 16846 Mouse Omim: 164160 Human SwissProt: P41159 Human SwissProt: P41160 Mouse SwissProt: Q28603 Sheep Unigene: 194236 Human Unigene: 277072 Mouse Unigene: 44444 Rat Leptin是一種分子量為16kDa的脂肪組織源激素，又稱：肥胖蛋白(Obese-protein)是由脂肪細胞分泌的，具有強親水性，以單體形式存在于血漿中。 Leptin 具有廣泛的生物學效應，作用于下丘腦，調節(jié)食欲、能量代謝及體重。Leptin 還可能作為脂肪-胰島內分泌軸的一部分，參與胰島素分泌的調節(jié)。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leptin) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-10888M 3C2) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leptin) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-10888M 3C2) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
