| 產(chǎn)品編號(hào) | bsm-51485M |
| 英文名稱 | Mouse Anti-EIF4E2 antibody |
| 中文名稱 | EIF4E2單克隆抗體 |
| 別 名 | 4E LP; 4EHP; 4ELP; EIF 4E type 2; eIF-4E type 2; EIF4 E2; EIF4E 2; EIF4E HOMOLOGOUS PROTEIN; EIF4E like cap binding protein; EIF4E like protein 4E LP; EIF4E like protein 4ELP; EIF4E type 2; EIF4E type 3; eIF4E-like protein 4E-LP; EIF4EL3; Eukaryotic translation initiation factor 4E family member 2; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E like 3; Eukaryotic translation initiation factor 4E member 2; Eukaryotic translation initiation factor 4E type 2; Eukaryotic translation initiation factor 4E-like 3; IF 4e; IF4E; IF4E2_HUMAN; mRNA cap binding protein 4EHP; mRNA cap binding protein type 3; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 3. |
| 研究領(lǐng)域 | 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | E3H9 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human EIF4E2. |
| 亞 型 | IgG1,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation factor 4E family (eIF4E) is comprised of three proteins that are involved in the early initiation of protein synthesis. eIF4E2 (eukaryotic translation initiation factor 4E family member 2), also known as 4EHP, IF4e, 4E-LP or EIF4EL3, is a ubiquitously expressed 245 amino acid protein. During early translation events, eIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap (a cotranscriptionally added structure that conveys mRNA stability and allows for efficient RNA processing), thus initiating the unwinding of mRNA secondary structures and facilitating mRNA-ribosome binding. eIF4E2 competes with eIF4E (member 1) for cap binding and, upon modification by the ubiquitin-like protein ISG15 (Interferon-induced 15 kDa protein), exhibits increased mRNA cap affinity. Function: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Post-translational modifications: Ubiquitinated by ARIH1, leading to its degradation by the proteasome. Similarity: Belongs to the eukaryotic initiation factor 4E family. SWISS: O60573 Gene ID: 9470 Database links: Entrez Gene: 9470 Human SwissProt: O60573 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: NCI-H460 cell lysates
Primary: Anti-EIF4E2 (bsm-51485M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
|
