mm1313亚洲精品,欧美俄罗斯40老熟妇,欧美日韩在线观看视频在线,亚洲欧美国产激情综合在线

Sample: Lane 1: Jurkat cell lysates Lane 2: Human brain tissue lysates Lane 3: Mouse brain tissue lysates Lane 4: Mouse lung tissue lysates Lane 5: Rat brain tissue lysates Primary: Anti-PPP2R1B (bsm-51604M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 60 kD

Paraformaldehyde-fixed, paraffin embedded (Human spleen sections ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R1B) Monoclonal Antibody, Unconjugated (bsm-51604M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PPP2R1B) monoclonal Antibody, Unconjugated (bsm-51604M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes,Alexa Fluor? 555 conjugated with Phalloidin(red) was used to stain the cell Cytoplasmic actin.DAPI (blue) was used to stain the cell nuclei.

Blank control:Jurkat. Primary Antibody (green line): Mouse Anti-PPP2R1B antibody (bsm-51604M) Dilution: 1:25; Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488 Dilution: 1:400 Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.