| 產品編號 | bsm-51606M |
| 英文名稱 | Mouse Anti-DPYSL5 antibody |
| 中文名稱 | 二氫嘧啶酶相關蛋白5單克隆抗體 |
| 別 名 | Collapsin response mediator protein 5; CRAM; CRMP 3 associated molecule; CRMP 5; CRMP3 associated molecule; Dihydropyrimidinase like 5; Dihydropyrimidinase related protein 5; DPYS L5; DPYSL 5; DPYSL5; DRP 5; DRP5; FLJ45383; Ulip 6; Ulip6; ULIP6 protein; DPYL5_HUMAN. |
| 研究領域 | 神經(jīng)生物學 細胞類型標志物 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | C3W1 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-4000,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human DPYL5. |
| 亞 型 | IgG1,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The collapsin response mediator protein (CRMP) family of proteins (also referred to as ULIP6) are a group of neurologic proteins that have been found to play key roles in growth cone guidance during neural development. CRMP5 has relatively low sequence homology with the other four members of the CRMP family. CRMP5 is expressed in the developing nervous system in an expression pattern that resembles CRMP2 and has a peak expression during the first postnatal week. Elevated levels of autoantibody to CRMP5 have been shown to be linked paraneoplastic autoimmune optic neuritis and other paraneoplastic neoplasms. Auto-immunity against CRMP5 expression (predominantly the N-terminal epitopes) is also being investigated as an oncological marker in the respiratory system and in the thymus. Subunit: Homotetramer, and heterotetramer with other DPYS-like proteins. Interacts with DPYSL2, DPYSL3 and DPYSL4. Subcellular Location: Cytoplasm. Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. SWISS: Q9BPU6 Gene ID: 56896 Database links: Entrez Gene: 56896 Human Entrez Gene: 65254 Mouse SwissProt: Q9BPU6 Human SwissProt: Q9EQF6 Mouse |
| 產品圖片 |
Sample:
Lane 1: SH-SY5Y cell lysates
Lane 2: Mouse brain tissue lysates
Lane 3: Rat brain tissue lysates
Primary: Anti-DPYSL5 (bsm-51606M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (DPYSL5) monoclonal Antibody, Unconjugated (bsm-51606M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
