| 產(chǎn)品編號 | bsm-51665M |
| 英文名稱 | Mouse Anti-PDXK antibody |
| 中文名稱 | 吡哆醛激酶單克隆抗體 |
| 別 名 | PDXK.1; PDXK_HUMAN; PKH; PNK; Pyridoxal kinase; Pyridoxamine kinase; Pyridoxine kinase; Vitamin B6 kinase. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | H8F01 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-4000,IHC-P=1:25,IHC-F=1:25,IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PDXK between 1-312 amino acids. |
| 亞 型 | IgG1,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Pyridoxal kinase belongs to the pyridoxine kinase family and phosphorylates vitamin B6, a step necessary for the conversion of vitamin B6 to pyridoxal 5’ phosphate (PLP), the active form of vitamin B6. PLP acts as a coenzyme and functions to maintain homeostasis. Pyridoxal kinase is a 312-amino acid cytoplasmic protein that may act as a homodimer and is expressed ubiquitously. There are three known isoforms of pyridoxal kinase, and isoform 3 expression is observed in adult testis and spermatozoa. The optimum pH for pyridoxal kinase is between 5.5 and 6.0. PDXK, the gene that encodes the pyridoxal kinase protein, maps to chromosome 21q22.3 and may be a candidate gene for autoimmune polyglandular disease type 1, a genetic disorder that has been mapped to the same region on chromosome 21. Function: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Subunit: Homodimer (Probable). Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Isoform 3 is detected in adult testis and spermatozoa. Similarity: Belongs to the pyridoxine kinase family. SWISS: O00764 Gene ID: 8566 Database links: Entrez Gene: 8566 Human SwissProt: O00764 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: 293 cell lysates
Lane 2: MCF-7 cell lysates
Lane 3: U-251 MG cell lysates
Lane 4: Human liver tissue lysates
Primary: Anti-PDXK (bsm-51665M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Paraformaldehyde-fixed, paraffin embedded (human testis tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDXK) Monoclonal Antibody, Unconjugated (bsm-51665M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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