| 產品編號 | bsm-51666M |
| 英文名稱 | Mouse Anti-GNMT antibody |
| 中文名稱 | 甘氨酸-N-甲基轉移酶單克隆抗體 |
| 別 名 | EC 2.1.1.20; Epididymis secretory sperm binding protein Li 182mP; Glycine N methyltransferase; Glycine N-methyltransferase; GNMT_HUMAN; HEL S 182mP; OTTHUMP00000016412. |
| 研究領域 | 信號轉導 新陳代謝 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | K8T2 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-4000,Flow-Cyt=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human GNMT between 1-295 amino acids. |
| 亞 型 | IgG2a,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GNMT catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). It plays a possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Function: Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Subunit: Homotetramer. Subcellular Location: Cytoplasm. Tissue Specificity: Abundant in liver. DISEASE: Defects in GNMT are the cause of glycine N-methyltransferase deficiency (GNMT deficiency) [MIM:606664]; also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Similarity: Belongs to the pyridoxine kinase family. SWISS: Q14749 Gene ID: 27232 Database links: Entrez Gene: 27232 Human SwissProt: Q14749 Human |
| 產品圖片 |
Sample:
Lane 1: Human liver tissue lysates
Primary: Anti-GNMT (bsm-51666M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 34 kD
Blank control:U-2 OS.
Primary Antibody (green line): Mouse Anti-GNMT antibody (bsm-51666M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG
Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution: 1:400
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
