| 產(chǎn)品編號 | bsm-51708M |
| 英文名稱 | Mouse Anti-PDCD1LG2 antibody |
| 中文名稱 | 程序性死亡配體2（CD273）單克隆抗體 |
| 別 名 | PD L2; PD-L2; PD1L2_HUMAN; B7 dendritic cell molecule; B7-DC; B7DC; bA574F11.2; Btdc; Butyrophilin B7 DC; Butyrophilin B7-DC; Butyrophilin B7DC; CD 273; CD273; CD273 antigen; MGC142238; MGC142240; PD 1 ligand 2; PD-1 ligand 2; PD1 ligand 2; PDCD 1 ligand 2; PDCD1 ligand 2; PDL 2; PDL2; Programmed cell death 1 ligand 2; Programmed death ligand 2. |
| 研究領(lǐng)域 | 免疫學(xué) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | G8F6 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-2000,IHC-P=1:100-400,IHC-F=1:100-400,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29kDa |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDCD1LG2: 31-130/273 |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Involved in negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; and negative regulation of interleukin-10 production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis. [provided by Alliance of Genome Resources, Nov 2021] Function: Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Subunit: Interacts with PDCD1. Subcellular Location: Isoform 3: Secreted (Probable). Isoform 2: Endomembrane system; Single-pass type I membrane protein. Isoform 1: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. Post-translational modifications: Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1. Similarity: Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9BQ51 Gene ID: 80380 Database links: Entrez Gene: 80380 Human Omim: 605723 Human SwissProt: Q9BQ51 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Non-transfected 293T cell lysates
Lane 2: Transfected PD-L2-transfected 293T cell lysates
Primary: Anti-PDCD1LG2 (bsm-51708M) at 1/8000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 40-55 kD
Immunohistochemical analysis of PDL-2 in untransfected(left) or transfected(right) with 293T cell sections. Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
