| 產(chǎn)品編號 | bsm-51738M |
| 英文名稱 | Mouse Anti-PURB antibody |
| 中文名稱 | 轉(zhuǎn)錄激活蛋白PURB單克隆抗體 |
| 別 名 | Pur beta; PURB_HUMAN; PURBETA; Purine rich element binding protein B; Purine-rich element-binding protein B; transcriptional activator protein Pur beta; Transcriptional activator protein Pur-beta. |
| 研究領(lǐng)域 | 染色質(zhì)和核信號 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | F4D9 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:1000-4000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PURB. |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008] Function: Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs. Subunit: Homodimer, heterodimer with PURA and heterotrimer with PURA and YBX1/Y-box protein 1. Subcellular Location: Nucleus. Tissue Specificity: Expressed in myocardium of heart failure patients. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the PUR DNA-binding protein family. SWISS: Q96QR8 Gene ID: 5814 Database links: Entrez Gene: 5814 Human Entrez Gene: 19291 Mouse SwissProt: Q96QR8 Human SwissProt: O35295 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Jurkat cell lysates
Lane 2: HeLa cell lysates
Lane 3: NCI-H460 cell lysates
Lane 4: HepG2 cell lysates
Lane 5: MCF-7 cell lysates
Lane 6: NIH/3T3 cell lysates
Primary: Anti-PURB (bsm-51738M) at 1/8000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 37 kD
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