| 產(chǎn)品編號 | bsm-54152R |
| 英文名稱 | MMP3 Recombinant Rabbit mAb |
| 中文名稱 | 基質(zhì)金屬蛋白酶3重組兔單抗 |
| 別 名 | MMP3_HUMAN; Stromelysin-1; EC:3.4.24.17; STMY1; SL-1; SL 1; SL1; Matrix metalloproteinase-3(MMP-3); MMP-3; MMP 3; Transin-1; |
| 研究領(lǐng)域 | 腫瘤 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP-3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]. Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Subcellular Location: Secreted, extracellular space, extracellular matrix (Probable). Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P08254 Gene ID: 4314 合成與降解（Synthesis and Degradation） 基質(zhì)金屬蛋白酶(matrix metalloproteinases, MMPs)是一族依賴鋅離子而降解各種細胞外基質(zhì)的蛋白酶，亦稱IV型膠原酶或稱明膠酶A，其主要功能為降解IV型膠原，因而它在腫瘤細胞突破基底膜屏障和浸潤轉(zhuǎn)移中起重要作用。 MMP3目前主要用于各種惡性腫瘤(如乳腺癌、胃腸道癌、卵巢癌、膀胱癌等)中的基底膜檢測與腫瘤轉(zhuǎn)移浸潤的研究。 |
| 產(chǎn)品圖片 |
Western blot analysis of MMP3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (bsm-54152R, 1/500) was used in 5% BSA at room temperature for 2 hours. Go
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at
room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: Raji, 2: U87-MG, 3: Mouse placenta,
4: Rat placenta
Protein loading quan
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MMP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 mi
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MMP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 mi
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-MMP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 min
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MMP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30
Tissue: Human liver
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at
ro
Cell line: HT-29
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is th
Cell line: NIH/3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is
Cell line: HT-29
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is th
Cell line: NIH/3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is
Cell line: HepG2
Fixation: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Is
Cell line: HepG2
Fixation: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabelled control: The cell without incubation
with primary antibody and secondary antibody
(Black line).
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
