| 產(chǎn)品編號 | bs-80295G-HRP |
| 英文名稱 | Goat Anti-Rabbit IgG H&L, HRP conjugated |
| 中文名稱 | 辣根過氧化物酶標記羊抗兔IgG (H+L) |
| 別 名 | Goat Anti-Rabbit IgG H&L /HRP; Immunoglobulin G; 辣根過氧化物酶標記二抗; 辣根過氧化物酶標記山羊抗兔IgG (H+L); HRP標記山羊抗兔IgG (H+L); HRP標記山羊抗兔IgG (H+L); |
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Specific References (6) | bs-80295G-HRP has been referenced in 6 publications.
[IF=10.588] Haiyang Zhang. et al. Co-exposure of chronic stress and alumina nanoparticles aggravates hippocampal microglia pyroptosis by activating cathepsin B/NLRP3 signaling pathway. J HAZARD MATER. 2022 Aug;436:129093 IHC ; Rat.
[IF=7.419] Abulaiti Abulizi. et al. Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway. BIOMED PHARMACOTHER. 2023 Apr;160:114330 WB ; Rat.
[IF=6.268] Guo, Xin. et al. The histone demthylase KDM3A protects the myocardium from ischemia/reperfusion injury via promotion of ETS1 expression. Commun Biol. 2022 Mar;5(1):1-15 WB ; Rat.
[IF=4.136] Xiaoyue Huang. et al. Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma. ONCOL REP. 2022 Jul;48(1):1-18 WB ; Human.
[IF=3.616] Shugen Xu. et al. Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringer’s solution. ANN TRANSL MED. 2022 Dec; 10(24): 1331 WB ; Rat.
[IF=2.586] Peng Zhang. et al. Effect of isorhamnetin on carbonic anhydrase IX expression and tumorigenesis of bladder cancer by activating PPARγ/PTEN/AKT pathway. TISSUE CELL. 2023 Jun;82:102048 IHC,WB ; Mouse,Human.
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| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rabbit |
| 產(chǎn)品應用 | WB=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1.0 mg/ml |
| 免 疫 原 | Native Rabbit IgG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed
HRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Spleen Lysates
Lane 2: Human SH-SY5Y cell Lysates
Primary: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Secondary: Goat Anti-Rabbit IgG H&L/HRP at 1/100000 dilution
Predicted band size: 42kDa
Observed band size: 45kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
