| 產(chǎn)品編號 | bs-60331R |
| 英文名稱 | Rabbit Anti-MUC2 antibody |
| 中文名稱 | 粘蛋白-2/上皮膜抗原2抗體 |
| 別 名 | MLP; SMUC; Intestinal mucin 2; MUC 2; MUC2; Mucin 2; Mucin 2 intestinal; Mucin 2 intestinal/tracheal; Mucin 2 oligomeric mucus/gel forming; Mucin 2 precursor; Mucin 2 tracheal; Mucin like protein; Intestinal mucin-2; MUC-2; MUC2_HUMAN; Mucin-2; Mucin2. |
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Specific References (2) | bs-60331R has been referenced in 2 publications.
[IF=5.561] Qianqian Yao. et al. The Milk Active Ingredient, 2′-Fucosyllactose, Inhibits Inflammation and Promotes MUC2 Secretion in LS174T Goblet Cells In Vitro. FOODS. 2023 Jan;12(1):186 IF ; Human.
[IF=3.337] Wang, Chengyao. et al. Causative Role of Anoxic Environment in Bacterial Regulation of Human Intestinal Function. CELL MOL BIOENG. 2022 Sep;:1-12 IF ; Human.
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| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 糖蛋白 細(xì)胞類型標(biāo)志物 腫瘤細(xì)胞生物標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:50-100,IHC-F=1:50-100,ICC/IF=1:50,IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 570kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MUC2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008] Function: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Subunit: Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization. Interacts with FCGBP. Interacts with AGR2; disulfide-linked. Subcellular Location: Secreted. Note=In the intestine, secreted into the inner and outer mucus layers. Tissue Specificity: Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. Post-translational modifications: O-glycosylated. May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. Similarity: Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 TIL (trypsin inhibitory-like) domain. Contains 2 VWFC domains. SWISS: Q02817 Gene ID: 4583 Database links: Entrez Gene: 4583 Human Entrez Gene: 17831 Mouse SwissProt: Q02817 Human SwissProt: Q80Z19 Mouse 細(xì)胞粘附蛋白（Call Adhesion Protein） 上皮膜抗原是粘性糖蛋白家族之一,是一種膜內(nèi)在糖蛋白。Muc-2表達(dá)僅限于內(nèi)膜腺上皮和腔上皮,很多上皮細(xì)胞及其來源的腫瘤表達(dá)該蛋白.其合成和分泌是腺上皮組織的特征之一。MUC-2也是一種主要的腸道粘蛋白，在正常腸道和結(jié)腸癌、胃癌等腫瘤中有廣泛分布。該抗體主要用于胃腸道腫瘤的研究。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC2) Polyclonal Antibody, Unconjugated (bs-60331R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC2) Polyclonal Antibody, Unconjugated (bs-60331R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Cell line: HT-29
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bs-60331R
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