| 產(chǎn)品編號 | bsm-54293R |
| 英文名稱 | Biglycan Recombinant Rabbit mAb |
| 中文名稱 | 骨/軟骨蛋白多糖1重組兔單抗 |
| 別 名 | BGN; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A. |
| 研究領(lǐng)域 | 細胞生物 免疫學 信號轉(zhuǎn)導 干細胞 細胞骨架 細胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant Human Biglycan |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
May be involved in collagen fiber assembly. Function: May be involved in collagen fiber assembly (By similarity). Subunit: Homodimer. Forms a ternary complex with MFAP2 and ELN Subcellular Location: Secreted, extracellular space, extracellular. Tissue Specificity: Found in several connective tissues, especially in articular cartilages. Post-translational modifications: The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity). Similarity: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats. SWISS: P21810 Gene ID: 633 Database links: Entrez Gene: 633 Human Entrez Gene: 12111 Mouse Omim: 301870 Human SwissProt: P21810 Human SwissProt: P28653 Mouse Unigene: 821 Human Unigene: 2608 Mouse Unigene: 783 Rat |
| 產(chǎn)品圖片 |
Western blot analysis of Biglycan on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (bsm-54293R, 1/500) was used in 5% BSA at room temperature for
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Biglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 m
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Biglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 mi
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
