| 產(chǎn)品編號(hào) | bsm-34218M |
| 英文名稱 | Mouse Anti-BRAF antibody |
| 中文名稱 | B Raf單克隆抗體 |
| 別 名 | BRAF_HUMAN; Serine/threonine-protein kinase B-raf; EC:2.7.11.1; BRAF1; RAFB1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; B-Raf proto-oncogene, serine/threonine kinase; NS7; B-raf; B-RAF1; BRAF-1; 94 kDa B raf protein; |
| 研究領(lǐng)域 | 腫瘤 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 激酶和磷酸酶 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:100-1000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:50-500,IP=1:100-500,ELISA=1:30-3000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human B-Raf protein: 12-156/766 |
| 亞 型 | IgG2a κ |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017] Subunit: Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Subcellular Location: Nucleus. Cytoplasm. Cell membrane. Tissue Specificity: Brain and testis. SWISS: P15056 Gene ID: 673 Database links: Entrez Gene: 673 Human Entrez Gene: 109880 Mouse SwissProt: P15056 Human SwissProt: P28028 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Human Raji cell lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human MOLT4 cell lysates
Lane 7: Human HL-60 cell lysates
Primary: Anti- B-Raf (bsm-34218M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 84(hu)/89(mo, rat) kDa
Observed band size: 95(hu)/100(mo, rat) kDa
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