| 產品編號 | bsm-60173M |
| 英文名稱 | Mouse Anti-Butyryl-Histone H4 (Lys5) antibody |
| 中文名稱 | |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | H12E10 |
| 交叉反應 | (predicted: Human,Mouse,Rat) |
| 產品應用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | Antigen affinity purification |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | 組蛋白經過各種酶催化的修飾，包括乙?；谆?，磷酸化，泛素化等。賴氨酸丁?；切掳l(fā)現(xiàn)的一種控制染色體結構和基因轉錄的可逆修飾。賴氨酸丁?；趶娜湎x到人的真核細胞中高度保守。組蛋白賴氨酸丁?；毺氐慕Y構和基因組定位表明，它在機制和功能上不同于組蛋白賴氨酸乙酰化。具體地說，在人類體細胞和兔雄性生殖細胞基因組中，組蛋白丁酰化標志著活性啟動子或潛在增強子。組蛋白H3在Lys18位點的丁?；赡茉诒碛^遺傳調控中發(fā)揮重要作用，包括染色質重塑和DNA轉錄調控。 |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Cell line: (A) HeLa, (B) HeLa+SBA (5mM, 24hr)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60173M
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
