| 產(chǎn)品編號 | bsm-60260M |
| 英文名稱 | Mouse Anti-ATR antibody |
| 中文名稱 | |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | A5B4 |
| 交叉反應(yīng) | (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG1/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 絲氨酸/蘇氨酸蛋白激酶，在基因毒性應(yīng)激（如電離輻射（IR）、紫外線（UV）或DNA復(fù)制停滯）時激活檢查點信號，從而充當(dāng)DNA損傷傳感器。識別底物共有序列[ST]-Q。磷酸化BRCA1、CHEK1、MCM2、RAD17、RPA2、SMC1和p53/TP53，共同抑制DNA復(fù)制和有絲分裂，促進(jìn)DNA修復(fù)、重組和凋亡。在DNA損傷部位磷酸化組蛋白變體H2AX的“Ser-139”，從而調(diào)節(jié)DNA損傷反應(yīng)機制。FANCD2泛素化所需。對維持脆弱位點的穩(wěn)定性和中心體復(fù)制的有效調(diào)控至關(guān)重要。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HeLa, MCF7, HepG2, A549, Jurkat
Protein loading quantity: 20 μg
Exposure time: 180 s
Predicted MW: 300 kDa
Observed MW: 240 kDa
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