| 產(chǎn)品編號 | bsm-60639M |
| 英文名稱 | Mouse Anti-GART antibody |
| 中文名稱 | 甘氨酰胺核苷酸合成酶單克隆抗體 |
| 別 名 | 5'-phosphoribosylglycinamide transformylase; AIR synthase; AIRS; GAR transformylase; GARS; GARTF; Glycinamide ribonucleotide synthetase; MGC47764; PAIS; PGFT; Phosphoribosyl-aminoimidazole synthetase; Phosphoribosylglycinamide formyltransferase; Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase; Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29; Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase; Phosphoribosylglycinamide synthetase; PRGS; PUR2_HUMAN; Trifunctional purine biosynthetic protein adenosine 3. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 新陳代謝 表觀遺傳學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 交叉反應(yīng) | Human (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 107kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GART |
| 亞 型 | IgG1,Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | PBS, Glycerol, BSA. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events. SWISS: P22102 Gene ID: 2618 Database links: Entrez Gene: 2618 Human Entrez Gene: 14450 Mouse Omim: 138440 Human SwissProt: P22102 Human SwissProt: Q64737 Mouse Unigene: 473648 Human Unigene: 4505 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human A431 cell lysates
Lane 3: Human HepG2 cell lysates
Primary: Anti-GART (bsm-60639M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 55,130 kDa
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L
(HRP)
Lysate: 1: HepG2, 2: HEK-293, 3: PC-12
Protein loading quantity: 20 ug
Exposure time: 30 s
Predicted MW: 40, 108 kDa
Observed MW: 52, 120 kDa
Cell line: HepG2
Fixation: 100% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C
overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60639M
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
