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Rabbit Anti-PDHA1  antibody (bsm-60685R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bsm-60685R
英文名稱 Rabbit Anti-PDHA1  antibody
中文名稱 Runx3重組兔單抗
別    名 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; ODPA_HUMAN; EC:1.2.4.1; PDHE1-A type I; pyruvate dehydrogenase E1 subunit alpha 1; PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; PDH-E1α;   
研究領(lǐng)域 線粒體  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 R9B7
交叉反應(yīng) Human (predicted: Mouse,Rat)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 43kDa
細(xì)胞定位 細(xì)胞漿 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PDHA1 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]


Subcellular Location:
Mitochondrion matrix.

Tissue Specificity:
Testis. Expressed in postmeiotic spermatogenic cells.

SWISS:
P08559

Gene ID:
5160

Database links:


Entrez Gene: 5160 Human

Entrez Gene: 18597 Mouse

Entrez Gene: 29554 Rat

SwissProt: P08559 Human

SwissProt: P35486 Mouse

SwissProt: P26284 Rat





產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: Mouse kidney, 3: Rat kidney Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 43 kDa Observed MW: 43 kDa
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