| 產品編號 | bsm-43034M |
| 英文名稱 | Mouse Anti-HAVCR2/TIM-3 antibody |
| 中文名稱 | T淋巴細胞膜蛋白3(CD366)單克隆抗體 |
| 別 名 | TIM-3; Tim 3; TIM3; TIMD-3; TIMD3; HAVR2_HUMAN; Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; CD366; |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 免 疫 原 | Recombinant human TIM-3 protein: 22-200/301 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011] Function: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Subcellular Location: Membrane. Tissue Specificity: T-helper type 1 lymphocyte (Th1)-specific. Post-translational modifications: O-glycosylated with core 1 or possibly core 8 glycans. Similarity: Belongs to the immunoglobulin superfamily. TIM family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q8TDQ0 Gene ID: 84868 Database links: Entrez Gene: 84868 Human SwissProt: Q8TDQ0 Human |
| 產品圖片 |
Sample:
Lane 1: Recombinant human TIM-3 protein, His (HEK293)(bs-43034P)
Primary: Anti-HAVCR2/TIM-3 (bsm-43034M) at 1/1000 dilution
Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific
Predicted band size: 33 kDa
Observed band size: 40 kDa
Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HAVCR2) Monoclonal Antibody, Unconjugated (bsm-43034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HAVCR2) Monoclonal Antibody, Unconjugated (bsm-43034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
