產品編號 | bsm-33888M |
英文名稱 | Mouse Anti-14-3-3 eta antibody |
中文名稱 | 14-3-3 eta單克隆抗體 |
別 名 | 14 3 3 protein eta; 14-3-3 eta antibody; 1433F_HUMAN; Brain protein 14-3-3, eta isoform; HGNC:12853; Protein AS1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide; YWHA 1; YWHA1; Ywhah; |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 4H1 |
交叉反應 | Human,Mouse,Rat |
產品應用 | WB=1:2000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 28kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant human 14-3-3 eta Protein. |
亞 型 | IgG1 |
純化方法 | affinity purified by Protein A |
緩 沖 液 | PBS, pH7.4, containing 0.02% Proclin300 as Preservative and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009] Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Tissue Specificity: Expressed mainly in the brain and present in other tissues albeit at lower levels. Similarity: Belongs to the 14-3-3 family. SWISS: Q04917 Gene ID: 7533 Database links: Entrez Gene: 7533 Human Entrez Gene: 22629 Mouse Omim: 113508 Human SwissProt: Q04917 Human SwissProt: P68510 Mouse Unigene: 226755 Human Unigene: 332314 Mouse Unigene: 409488 Mouse Unigene: 3324 Rat |
產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Lane 5: Human 293T cell lysates
Primary: Anti-14-3-3 eta (bsm-33888M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |