| 產(chǎn)品編號(hào) | bsm-42319M |
| 英文名稱 | Mouse Anti-Human Lambda chain antibody |
| 中文名稱 | 人λ鏈單克隆抗體 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 2F3 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 免 疫 原 | Purified native protein |
| 亞 型 | IgG1 |
| 緩 沖 液 | 0.01M PBS (pH7.4). |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. Subcellular Location: Cytoplasmic Similarity: Contains 1 Ig-like (immunoglobulin-like) domain. SWISS: P0CG04 Gene ID: 3535 Database links: Entrez Gene: 3535 Human Entrez Gene: 3546 Human Entrez Gene: 8217 Human Omim: 147220 Human SwissProt: P01701 Human SwissProt: P01842 Human 該抗體特異識(shí)別免疫球蛋白λ輕鏈，可用于鑒定白血病、漿細(xì)胞瘤以及某些非霍奇金氏淋巴瘤。這些腫瘤的共同特征是只限于單個(gè)輕鏈的表達(dá)，因此輕鏈Kappa和Lamda可用于良惡性淋巴病變（反應(yīng)性淋巴細(xì)胞增生和淋巴瘤）的輔助性診斷。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human Serum
Primary: Anti-Human Lambda chain (bsm-42319M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 25 kDa
Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (bsm-42319M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (bsm-42319M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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