產(chǎn)品編號(hào) | bs-0646R-Cy5 |
英文名稱1 | Rabbit Anti-CD34/Cy5 Conjugated antibody |
中文名稱 | Cy5標(biāo)記的CD34抗體 |
別 名 | CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN. |
Journal
PMID
IF
Application
[IF=7.59] Shih-Ying Sung. et al. Supercritical carbon dioxide-decellularized arteries exhibit physiologic-like vessel regeneration following xenotransplantation in rats. BIOMATER SCI-UK. 2023 Feb;: IF ; Rat.
[IF=6.081] Yi-Wen Lin. et al. Far-Infrared Therapy Decreases Orthotopic Allograft Transplantation Vasculopathy. BIOMEDICINES. 2022 May;10(5):1089 FC ; Rat.
[IF=4.069] Feng-Yen Linet al. Dipeptidyl Peptidase-4 Inhibitor Decreases Allograft Vasculopathy Via Regulating the Functions of Endothelial Progenitor Cells in Normoglycemic Rats. Cardiovasc Drugs Ther
. 2020 Jul 4. FCM ; rat.
[IF=3.426] Chien-Sung Tsai. et al. Transferring Plasmon Effect on a Biological System: Expression of Biological Polymers in Chronic Rejection and Inflammatory Rat Model. Polymers-Basel. 2021 Jan;13(11):1827 FC ; Rat.
[IF=2.72] Li, Guanghui, et al. "Coculture of peripheral blood CD34+ cell and mesenchymal stem cell sheets increase the formation of bone in calvarial critical-size defects in rabbits." British Journal of Oral and Maxillofacial Surgery (2013). Rabbit.
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規(guī)格價(jià)格 | 100ul/2980元 購買 大包裝/詢價(jià) |
說 明 書 | 100ul |
研究領(lǐng)域 | 腫瘤 心血管 免疫學(xué) 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 細(xì)胞表面分子 糖蛋白 細(xì)胞類型標(biāo)志物 血管內(nèi)皮細(xì)胞 內(nèi)皮細(xì)胞 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, ) |
產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 39kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CD34 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. Function: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Post-translational modifications: Highly glycosylated. Phosphorylated on serine residues by PKC. Similarity: Belongs to the CD34 family. Database links: Entrez Gene: 947 Human Omim: 142230 Human SwissProt: P28906 Human Unigene: 374990 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 造血干細(xì)胞標(biāo)志物 內(nèi)皮標(biāo)志物 腫瘤生物標(biāo)志物 細(xì)胞表面的唾液粘蛋白。 間充質(zhì)干細(xì)胞(mesenchymal stem cells,MSC)也是干細(xì)胞家族的重要成員,來源于發(fā)育早期的中胚層和外胚層。MSC最初在骨髓中發(fā)現(xiàn),因其具有多向分化潛能、造血支持和促進(jìn)干細(xì)胞植入、免疫調(diào)控和自我復(fù)制等特點(diǎn)。如間充質(zhì)干細(xì)胞在體內(nèi)或體外特定的誘導(dǎo)條件下,可分化為血管內(nèi)皮、脂肪、骨、軟骨、肌肉、肌腱、韌帶、神經(jīng)、肝、心肌、等多種組織細(xì)胞。 |
產(chǎn)品圖片 |
Blank control: HUVEC.
Primary Antibody (green line): Rabbit Anti-CD34 antibody (bs-0646R-CY5)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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