| 產(chǎn)品編號(hào) | bs-3125R-Gold |
| 英文名稱(chēng)1 | Rabbit Anti-phospho-Cyclin E1 (Thr77)/Gold Conjugated antibody |
| 中文名稱(chēng) | 膠體金標(biāo)記的磷酸化周期素E1抗體 |
| 別 名 | CCNE 1; CCNE;CCNE1;Cyclin E1 antibody Cyclin Es;Cyclin Et;CyclinE; G1/S specific cyclin E; CCNE1_HUMAN. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買(mǎi) 大包裝/詢(xún)價(jià) |
| 說(shuō) 明 書(shū) | 100ul（10nm 15nm 35nm） |
| 產(chǎn)品類(lèi)型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 細(xì)胞周期蛋白 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 47kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Function: Essential for the control of the cell cycle at the G1/S (start) transition. Subunit: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Post-translational modifications: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. Similarity: Belongs to the cyclin family. Cyclin E subfamily. Database links: Entrez Gene: 898 Human Entrez Gene: 12447 Mouse Omim: 123837 Human SwissProt: P24864 Human SwissProt: Q61457 Mouse Unigene: 244723 Human Unigene: 16110 Mouse Unigene: 15455 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ????細(xì)胞周期素E是調(diào)控細(xì)胞G-1→S期轉(zhuǎn)變的關(guān)鍵因素。由于在多種腫瘤中的不適當(dāng)表達(dá),細(xì)胞周期素E現(xiàn)在已明確為原癌基因。 |
