產(chǎn)品編號 | bsm-0287M-Cy3 |
英文名稱1 | Mouse Anti-His tag(2D5)/Cy3 Conjugated antibody |
中文名稱 | Cy3標記的小鼠抗聚組氨酸單克隆抗體 |
別 名 | HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
產(chǎn)品類型 | 標簽抗體 |
研究領域 | 細胞生物 免疫學 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 2D5 |
交叉反應 | Recombinant protein |
產(chǎn)品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated HHHHHH peptide |
亞 型 | IgG |
純化方法 | affinity purified by Protein G |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 蛋白質(zhì)純化的新途徑─應用His-Tag抗體檢測His-Tag融合蛋白 1) 6xHis與不同的Sepharose純化填料結合(捆綁),可不受構象的影響,在天然或變形的條件下可進行進一步純化。 2) 可在溫和的洗脫液環(huán)境中,其結合、洗滌及洗脫步驟不會對蛋白結構產(chǎn)生影響。 3) 6xHis標簽比普通(Tag)要小得多,經(jīng)純化后的蛋白可直接進行下游操作??捎糜谌魏伪磉_系統(tǒng)(包括: 細菌,桿狀病毒和哺乳動物) 4) 6xHis標簽在生理溶液pH下不帶電荷,其標簽不會干涉重組蛋白的結構和功能, 6xHis不會影響蛋白分泌。 5) 6xHis標簽免疫原性差,重組蛋白可以不必除去標簽而直接作為抗原進行免疫。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |