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Rabbit Anti-Tubulin-alpha/Biotin Conjugated antibody (bs-0159R-Bio)
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說(shuō) 明 書(shū): 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-0159R-Bio
英文名稱 Rabbit Anti-Tubulin-alpha/Biotin Conjugated antibody
中文名稱 生物素標(biāo)記的微管蛋白α/Tubulin α抗體
別    名 Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A; α-tubulin; α tubulin.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買(mǎi)        大包裝/詢價(jià)
說(shuō) 明 書(shū) 100ul  
研究領(lǐng)域
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat,  (predicted: Cow, Sheep, )
產(chǎn)品應(yīng)用 WB=1:50-200 ELISA=1:100-1000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 50kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Tubulin-alpha 1
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Tubulin is a major cytoskeleton that has five distinct forms, designated alpha, beta, gamma, delta and epsilon tubulin. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin bind GTP. Only beta tubulin hydrolyzes GTP to GDP. This hydrolysis is a process that is linked to tubulin polymerization and microtubule formation. The alpha tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.

Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit:
Dimer of alpha and beta chains.

Subcellular Location:
Cytoplasm, cytoskeleton.

Post-translational modifications:
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Similarity:
Belongs to the tubulin family.

Database links:

Entrez Gene: 7846 Human

Entrez Gene: 22142 Mouse

Entrez Gene: 64158 Rat

Omim: 602529 Human

SwissProt: Q71U36 Human

SwissProt: P68369 Mouse

SwissProt: P68370 Rat

Unigene: 654422 Human

Unigene: 405359 Mouse

Unigene: 234326 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

結(jié)構(gòu)蛋白(Structural Proteins)
tubulin是一種大量存在于哺乳動(dòng)物腦組織中的微管亞基蛋白,在結(jié)構(gòu)上是由兩個(gè)極為相近的α和β亞基組成的二聚體、多聚體形成微管細(xì)絲,是微管的主要成分。
微管蛋白是球形分子, 有兩種類(lèi)型:α微管蛋白(α-tubulin)和β微管蛋白(β-tubulin)貨號(hào):bs-0210R, 這兩種微管蛋白具有相似的三維結(jié)構(gòu), 能夠緊密地結(jié)合成二聚體, 作為微管組裝的亞基。
α亞基由450個(gè)氨基酸組成, β亞基是由455個(gè)氨基酸組成, 這兩種亞基有35~40%的氨基酸序列同源, 表明編碼它們的基因可能是由同一原始祖先演變而來(lái).
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