| 產(chǎn)品編號 | bsm-33111M-HRP |
| 英文名稱1 | Mouse Anti-CD15/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的階段特異性胚胎表面抗原-1/CD15單克隆抗體 |
| 別 名 | Fut4; SSEA-1; 3-FAL; Alpha (1,3) fucosyltransferase; Alpha 13 fucosyltransferase FucT; EC 2.4.1.; ELAM 1 ligand fucosyltransferase; ELAM ligand fucosyltransferase; ELAM1 ligand fucosyltransferase; ELFT; FCT3A; Fuc TIV; Fucosyltransferase 4 alpha 1 3 fucosyltransferase myeloid specific; Fucosyltransferase 4; FucT IV; FUCTIV; FUT4; Galactoside 3 L fucosyltransferase; Lewis X; LeX; SSEA 1; SSEA1; Stage specific embryonic antigen 1; FUC-TIV. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 細胞膜受體 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 8B9 |
| 交叉反應(yīng) | (predicted: Human, ) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 58kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD15 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 儲 存 液 | Constituents: 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin, 50% glycerol. Or Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin. Reconstitute with sterile distilled water. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]. Function: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. Subcellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. Tissue Specificity: Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas. Similarity: Belongs to the glycosyltransferase 10 family. Database links: Entrez Gene: 10690 Human Entrez Gene: 2526 Human Entrez Gene: 2527 Human Entrez Gene: 2528 Human Entrez Gene: 2529 Human Omim: 104230 Human SwissProt: P22083 Human SwissProt: P51993 Human SwissProt: Q11128 Human SwissProt: Q11130 Human SwissProt: Q9Y231 Human Unigene: 390420 Human Unigene: 457 Human Unigene: 49117 Human Unigene: 572064 Human Unigene: 623098 Human Unigene: 631843 Human Unigene: 631846 Human Unigene: 705615 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD15(SSEA-1)是胚胎干細胞表達胚胎階段特異性抗原的主要SSEA-1蛋白,還包括、SSEA-3、TRA-1-81、TRA-1-60等。CD15主要存在于粒細胞（包括中性粒細胞和嗜酸性粒細胞）以及部分單核細胞上。它在介導(dǎo)細胞吞噬細菌和趨化性方面起重要作用。CD15常表達于霍奇金氏病中R-S細胞，尤其是經(jīng)典型霍奇金淋巴瘤中。結(jié)節(jié)硬化型霍奇金淋巴瘤多為CD15陽性。CD15抗體是鑒定霍奇金氏病的一個有效工具。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
