| 產(chǎn)品編號 | bs-1150R |
| 英文名稱 | Rabbit Anti-muscarinic Acetylcholine Receptor 1 antibody |
| 中文名稱 | 毒蕈堿型乙酰膽堿受體M1抗體 |
| 別 名 | muscarinic acetylcholine receptor; M-AChR M1; Muscarinic Acetylcholine Receptor 1;cholinergic receptor, muscarinic 1; Chrm1; Chrm-1; M1; M1R; Muscarinic Acetylcholine receptor M1; Cholinergic receptor muscarinic 1; CHRM 1; CHRM1; HM 1; HM1; M1; M1 muscarinic cholinergic receptor; MGC30125; Muscarinic acetylcholine receptor M1; Acetylcholine receptor(M1). |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 細胞膜受體 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human muscarinic Acetylcholine Receptor 1: 321-420/460 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]. Function: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Subunit: Interacts with GPRASP2. Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily. SWISS: P11229 Gene ID: 1128 Database links: Entrez Gene: 1128 Human Entrez Gene: 12669 Mouse Omim: 118510 Human SwissProt: P11229 Human SwissProt: P12657 Mouse AChR M1 毒蕈堿型乙酰膽堿受體M1,屬于G蛋白偶聯(lián)受體家族成員，主要分布于中樞神經(jīng)系統(tǒng)和周圍神經(jīng)系統(tǒng)，乙酰膽堿與受體結(jié)合后誘導(dǎo)其功能：抑制酰苷酸環(huán)化酶、降解磷酸肌醇、膜鉀離子通道開放. |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-muscarinic Acetylcholine Receptor 1 (bs-1150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 61 kD
Sample:
Kidney(Rat) lysate at 30ug;
Brain(Rat) lysate at 30ug;
Primary: Anti-ChRM1/Acetylcholine receptor(M1) (bs-1150R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 51kD
Observed band size : 51kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
