| 產(chǎn)品編號 | bs-0508R |
| 英文名稱 | Rabbit Anti-PRL antibody |
| 中文名稱 | 泌乳素抗體 |
| 別 名 | Luteotropic Hormone; Mammotropin; OTTHUMP00000017727; PRL; Prolactin; Prolactin precursor; PRL_HUMAN. |
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Specific References (5) | bs-0508R has been referenced in 5 publications.
[IF=3.32] Hu, Bin, et al. "IFN?\?? Inhibits Osteopontin Expression in Human Decidual Stromal Cells and can be Attenuated by 1??, 25?\Dihydroxyvitamin D3."rican Journal of Reproductive Immunology 68.4 (2012): 353-361..rch Human.
[IF=2.425] Xie W et al. Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus). Eur J Histochem. 2019 Jan 17;63(1). IHC-P ; muskrat.
[IF=1.785] An J et al. A clinical and basic study of optimal endometrial preparation protocols for patients with infertility undergoing frozen?thawed embryo transfer. Exp Ther Med
. 2020 Sep;20(3):2191-2199. WB ; Human.
[IF=1.785] Junxia An. et al. A clinical and basic study of optimal endometrial preparation protocols for patients with infertility undergoing frozen?thawed embryo transfer. Exp Ther Med. 2020 Sep;20(3):2191-2199 WB ; Human.
[IF=0.352] Xu, Qian, et al. "CYCLIN G1 INHIBITS THE PROLIFERATION OF MOUSE ENDOMETRIAL STROMAL CELL IN DECIDUALIZATION."Arch Biol Sci. 2017;69(1):71-81. IF(ICC) ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 生長因子和激素 激酶和磷酸酶 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Chicken,Anser) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRL: 165-227/227 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]. Function: Prolactin acts primarily on the mammary gland by promoting lactation. Subcellular Location: Secreted. Similarity: Belongs to the somatotropin/prolactin family. SWISS: P01236 Gene ID: 5617 Database links: Entrez Gene: 5617 Human Entrez Gene: 19109 Mouse Omim: 176760 Human SwissProt: P01236 Human SwissProt: P06879 Mouse
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| 產(chǎn)品圖片 |
Sample:
A549 cell(human) Lysate at 40 ug
Primary: Anti-PRL (bs-0508R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24kD
Observed band size: 30 kD
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PRL Polyclonal Antibody, Unconjugated(bs-0508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PRL Polyclonal Antibody, Unconjugated(bs-0508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
